FHIT对急性髓系白血病细胞HL60影响的研究

目的:进行外源FHIT基因转染人白血病细胞缺乏FHIT表达的HL60,研究FHIT基因对转染细胞生长的生物学影响。方法:构建pEGFP-FHIT真核表达质粒(实验组)与质粒pEGFP-N1(对照组)。分别电穿孔法转染HL60细胞。应用荧光显微镜、RT-PCR、Westernblot检测转染基因的转录和表达情况。四甲基偶氮唑盐(MTT)法、流式细胞术研究转染基因对HL60细胞体外增殖、凋亡情况的影响,并与转染对照质粒的细胞株进行比较。结果:经PCR、酶切和DNA测序证实pEGFP-FHIT真核载体构建成功。荧光显微镜下实验组HL60细胞可见绿色荧光,转染率为40%。RT-PCR和Westernblot分别从mRNA和蛋白水平检测到FHIT的表达。MTT检测结果显示:转染后实验组抑制率明显升高,细胞增殖受到抑制(P<0.05)。流式细胞术结果显示:实验组细胞凋亡率明显增加(P<0.05)。结论:转染FHIT基因对白血病细胞HL60的生长有抑制作用,并能诱导其凋亡。

Findings in primary hepatic carcinoma perfusion and related factors by total liver perfu-sion CT

Objective：To investigate hePatocellular carcinoma and Peritumoral tissue Perfusion characteristics and related factors by the total liver Perfusion CT. Methods：All 56 Patients diagnosed with Primary hePatic carcinoma had accePted the total liver CT Perfusion scanning. eePatic artery Perfusion（eAP）and hePatic Perfusion index（ePI）of the Perfusion Pseudo - color Pictures were obtained. Target images showing maximum diameter of the tumor surface were selected in dynamic enhanced CT images. By measuring tumor diameter（D）and necrosis diameter（d）,the de-gree of tumor necrosis index R（R = d/ D × 100% ）was calculated. In the target images,ROIs were set on tumor tissue, Peritumoral tissue of 1 - 2cm area,non - tumor tissue. The eAP,ePI of different ROIs were obtained,which were used to analyze the correlation between tumor Perfusion Parameters and the degree of tumor necrosis,the correlation between tumor size,tumor necrosis and Peritumoral tissue Perfusion Parameters. Results：The eAP,ePI of tumor tis-sue was higher than non - tumor tissue in cases of PeC（P ﹤ 0. 05）. The eAP of tumor tissue was significantly nega-tively correlated（r = - 0. 721,P ﹤ 0. 05）with the tumor necrosis index,while there was no correlation between the ePI and the tumor necrosis index（P = 0. 244）. The eAP,ePI of Peritumoral tissue was higher than non - tumor tis-sue（P ﹤ 0. 05）. The eAP,ePI of tumor tissue was significantly Positive correlated（r = 0. 489,0. 544,P ﹤ 0. 05）with the tumor diameter,and tumor necrosis factor showed synergistic effect. Conclusion：Total liver Perfusion imaging with MSCT is valuable to evaluate the Perfusion characteristics of hePatocellular carcinoma,and may reflect the imPact of tumor diameter,tumor necrosis to the Peritumoral tissue Perfusion.