中国6省莱姆病螺旋体主要宿主动物鼠的初步调查

目的了解中国吉林、山西、甘肃、青海、贵州、湖南6省林区莱姆病螺旋体宿主动物鼠的感染情况。方法在每个省各选取两个采样点进行捕鼠,采用病原分离培养和巢式PCR方法对野鼠的脾脏、肾脏和膀胱进行了病原学检测。并通过基因测序方法确定基因型。结果从贵州黑线姬鼠中分离到了2株莱姆病螺旋体;从5省(贵州未检测到)野鼠的脾脏和/或肾脏中检查到了莱姆病螺旋体的特异片段,其中青海黄南(28.85%)和湖南石门(19.6%)两地标本的PCR阳性率较高,各地区阳性率的差异有统计学意义。通过序列同源性分析确定吉林、青海、甘肃和山西的基因型均为Borrelia garinii。湖南的基因型为Borreliavalaisiana。结论本次调查表明各地宿主动物鼠的感染状况不同,各地应根据具体情况进一步扩大调查以明确当地的主要宿主动物种类及携带病原体情况。

Rats, the primary reservoir hosts of Borrelia burgdorferi, in six representative provinces ,China

The objective of this study was to understand the status of rats,the reservoir hosts of Borrelia burgdorferi,in the six representative provinces in China including Jilin,Shanxi,Gansu,Qinghai,Guizhou and Hunan.The rats were catched in two different areas in each province and the pathogens were isolated and cultured.Nested-PCR was used to test Borrelia burgdorferi in spleen,kidney and bladder of rats.The sequence analysis was used to identify the genotype.Results indicated that two strains of Borrelia burgdorferi were obtained from apodemus in Guizhou;the specific fragments of Borrelia burgdorferi were obtained in spleen and kidney of rats from all representative provinces in China expect Guizhou;the PCR positive rate was higher in Huangnan（28.85%）and Shimen（19.6%）;there were statistical differences in the areas.The sequence analysis shows that the genotype in Jilin,Qinghai,Gansu and Shanxi belongs to Borrelia garinii,and in Hunan belongs to Borrelia valaisiana.The results indicated that there were different infection rate in rats infected in the six provinces,thus further investigation should conduct for the primary reservoir hosts and for the pathogens carrying situation in different areas of China.