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正交设计优选地龙的提取工艺
引用本文:卢欢,朱和平,严国俊,潘金火. 正交设计优选地龙的提取工艺[J]. 现代中药研究与实践, 2009, 23(1): 58-61
作者姓名:卢欢  朱和平  严国俊  潘金火
作者单位:南京中医药大学药学院,江苏,南京,210046;南京中医药大学药学院,江苏,南京,210046;南京中医药大学药学院,江苏,南京,210046;南京中医药大学药学院,江苏,南京,210046
摘    要:目的优选地龙的最佳提取工艺。方法通过琼脂纤维蛋白平板法测量溶圈面积,优选出用水匀浆法作为地龙的提取方法;综合考虑得率和溶圈面积这两个指标,采用正交实验对地龙的水匀浆工艺条件进行优化。结果最佳工艺参数为:即加入5倍量水,浸渍2h,以10000r/min转速在40℃下匀浆6h。结论优选出的工艺稳定可行。

关 键 词:地龙  提取工艺  正交实验

Study on Extract Technology of Pheretima by Orthogonai Design
LU Huan,ZHU He-ping,YAN Guo-jun,PAN Jin-huo. Study on Extract Technology of Pheretima by Orthogonai Design[J]. Research and Practice on Chinese Medicines, 2009, 23(1): 58-61
Authors:LU Huan  ZHU He-ping  YAN Guo-jun  PAN Jin-huo
Abstract:Objective To optimize the extract process of Pheretima .Methods The index of the extracting rate of Pheretima was evaluated by measuring the circular area using the fibrin-plate method.Results The optimum technology parameters are as follows:added 5 times amounts water , steeping 2 hours in water , then the homogenate for 6 hr with 10 000r/min rotation rate at 40℃ .Conclusion The optimized process is stable and highly efficient.
Keywords:Pheretima  extract technology  orthogonal design
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