实体瘤间期细胞制备技术对荧光原位杂交效果的影响

目的将改进的实体瘤间期细胞制备方法应用于荧光原位杂交,为实体瘤间期细胞的制备建立技术平台。方法采用随机引物法标记3号染色体着丝粒探针,分析实体瘤不同间期细胞制备技术对荧光原位杂交效果的影响。结果6种制片方法各具特点,根据其在应用方面有不同侧重,可采用不同制片方法。胶原酶法制片的荧光原位杂交效果最佳。对于冰冻组织或较小的组织,可采用印片法制片。结论适当的制片方法,结合荧光原位杂交,为实体瘤的染色体研究和诊断应用奠定了技术基础。

Influence of interphase nuclei preparation techniques on fluorescence in situ hybridization results in solid tumors

Objective To establish a technology platform for the preparation of interphase nuclei for the fluorescence in situ hybridization (FISH) detection of solid tumor tissues. Methods The centromere probe of chromosome 3 was labeled by the random primer technique, and then hybridized to interphase nuclei prepared by six different methods in order to study the influence on FISH detection. Results Each method of slide preparation had its own characteristic, and could be used according to different needs. As regards to FISH, collagenase method got the best results. Whereas for frozen samples or small tissues, to prepare printing slides was more applicable. Conclusion The comparison of different slide preparation methods lays a technology foundation for the FISH application in cancer researches and clinical diagnosis of solid tumors.