HPLC法测定益母草颗粒（无蔗糖型）中盐酸水苏碱的含量

目的：建立益母草颗粒（无蔗糖型）中盐酸水苏碱含量的测定方法。方法：采用反相高效液相色谱法，Ultimate Column XB—NH2（250mm×4．6mm，5μm）为色谱柱，乙腈-水（90：10）为流动相，检测波长为192nm，流速为1．0ml/min。结果：盐酸水苏碱在0．964—19．280μg范围内与峰面积呈良好线性关系（r=0．9993），盐酸水苏碱平均加样回收率为97．7％，RSD为1．23％（n=6）。结论：本方法可靠性高，准确性和重复性好，适用于益母草颗粒（无蔗糖型）中盐酸水苏碱的含量测定。

Content determination of Stachydrine Hydrochloride in Herba Leonuri Granules (sugar free) by HPLC

Objective： To develop a method for determination of Stachydrine Hydrochloride in Herba Leonuri Granules （sugar free）. Methods： The analytical column of reversed-phase high performance liquid chromatography （HPLC） method was Ultimate Column XB-NH2 （250 min×4.6 mm, 5 μm）. The mobile phase was a mixture of acetonitrile-water （90：10）, the flow rate was 1 ml/min. The detector wavelength was set at 192 nm. Results： The calibration curve was linear over the range of 0.964-19.280 μg for Staehydrine Hydrochloride （r=0.999 3）. The average recovery of Stachydrine Hydrochloride was 97.7%, RSD=1.23% （n=6）. Conclusion： The method is convenient, economical, it is adapted for use in the quantitative analysis of Stachydrine Hydrochloride.