上海市浦东新区2011年病毒性腹泻检测情况分析

目的了解上海市浦东新区病毒性腹泻的感染情况。方法采用实时荧光定量-聚合酶链式反应对2011年7—12月间上海市浦东新区12家监测医院的1327例腹泻患者的粪便标本同时进行诺如病毒、轮状病毒、星状病毒、扎如病毒和肠道腺病毒核酸检测。结果 1327份粪便样本中,179份样本检出至少1种病毒核酸,核酸阳性检出率为13.49%。诺如病毒、轮状病毒、扎如病毒、星状病毒以及肠道腺病毒的检出率分别为8.29%、2.79%、1.36%、1.21%和0.53%。各年龄组均有病毒检出,其中年龄最小的为2岁,最大为87岁,分别感染轮状病毒和诺如病毒。不同年龄组的病毒性腹泻感染没有差异。10月份开始是病毒性腹泻的高发期,各病毒的检出率均有明显升高。不同区域间腹泻病毒的感染没有差异。结论诺如病毒和轮状病毒是浦东地区病毒性腹泻的主要病原。应该加强对病毒性腹泻的监测,尤其是诺如病毒和轮状病毒。

Detection and Analysis of Viral Diarrhea in Pudong New District, Shanghai, 2011

OBJECTIVE To understand the incidenece of viral diarrhea in Pudong New District,Shanghai in 2011.METHODS A total 1327 stool samples collected from diarrhea patients in 12 hospitals to detect norovirus,rotavirus,sapovirus,astrovirus and enteric adenovirus by using real-time polymerase chain reaction（real-time PCR）.RESULTS 179 samples were detected more than one virus.The total detection rate was 13.49%,and which of norovirus,rotavirus,sapovirus,astrovirus and enteric adenovirus was 8.29%,2.79%,1.36%,1.21% and 0.53%,respectively.Virus positive samples were detected in all age group.The youngest and oldest patient was 2 and 87 years old and infected by rotavirus and norovirus.There was no difference in all age group to infected viral diarrhea.There was a peak in viral diarrhea infection from October and all detection rates were getting higher.There was also no difference of people in different places to infected viral diarrhea.CONCLUSION Norovirus and rotavirus were the major pathogens to cause the viral diarrhea in Pudong.It’s necessary to strengthen the surveillance of viral diarrhea,especially norovirus and rotavirus infections.