| 用多聚酶链反应检测LT-大肠杆菌 |
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| 引用本文： | 崔英霞,杨毓华. 用多聚酶链反应检测LT-大肠杆菌[J]. 医学研究生学报, 1992, 0(4) |
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| 作者姓名： | 崔英霞 杨毓华 |
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| 作者单位： | 全军医学检验中心微生物科，全军医学检验中心微生物科 |
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| 基金项目： | 全军“八五”医药卫生科研基金 |
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| 摘 要： | 本文报告用多聚酶链反应检测产生不耐热肠毒素大肠杆菌,使用二个寡核苷酸引物,直接从麦康凯平板的菌落中对该毒素的 A 亚基一段高度保守区域的 DNA 进行扩增,经琼脂糖凝胶电泳检测,30株细菌只有产生不耐热肠毒素大肠杆菌检测结果为阳性,其它细菌均为阴性.此法简便、特异、敏感.
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| 关 键 词： | 产生不耐热肠毒素大肠杆菌 不耐热肠毒素 多聚酶链反应 A 亚基 |
Detection of heat-labile-toxin of enterotoxigenic Eschericha Coli by polymerase chain reaction |
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| Cui Yingxi,et al. Detection of heat-labile-toxin of enterotoxigenic Eschericha Coli by polymerase chain reaction[J]. Bulletin of Medical Postgraduate, 1992, 0(4) |
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| Authors: | Cui Yingxi et al |
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| Affiliation: | Department of Microbiology |
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| Abstract: | This report described a polymerase chain reaction procedure for identification of heat-labile- toxin producing Escherichia Coli.Two oligonuclotido primers were used in this procedure to amplify a highly conserved region of the A subunit of the heat-labile enterotoxin gone.Amplification was done directly on E.Coil colonies from Macconkey plates.Detection of the amplified product can be done by agarose gel electrophoresis.30 strains were detected.Only heat-labile-toxin-producting Eschericha Coli were showed positive result,the rest of the strains were presented negative result. This method is simple,specific,sensitive for routine diagnosis of this pathosen in clinical isolates. |
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| Keywords: | heat-labile-toxin polymerase chain reaction A subunit |
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