| 高纯度平阳霉素铜螯合物纯化工艺研究 |
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| 引用本文： | 李亚军,李继安,张建斌,郭瑞玲,林惠敏. 高纯度平阳霉素铜螯合物纯化工艺研究[J]. 中国医药工业杂志, 2020, 0(3): 340-347 |
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| 作者姓名： | 李亚军 李继安 张建斌 郭瑞玲 林惠敏 |
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| 作者单位： | 中国医药工业研究总院上海医药工业研究院 |
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| 摘 要： | 本试验采用单分散聚合物微球填料柱色谱法纯化平阳霉素铜螯合物粗品,使其HPLC纯度高于98%。首先采用XR 3SP微球反相色谱纯化,使纯度和单步收率分别提高至50%和85%以上。然后经过POlyRP 30-300反相色谱纯化,纯度和单步收率可分别提高至98%和88%以上,总收率达75%以上。该工艺简单可靠,适于工业化生产。
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| 关 键 词： | 平阳霉素铜螯合物 反相色谱 分离纯化 |
Purification Process of High-purity Pingyangmycin Chelating Copper |
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| LI Yajun,LI Ji'an,ZHANG Jianbin,GUO Ruiling,LIN Huimin. Purification Process of High-purity Pingyangmycin Chelating Copper[J]. , 2020, 0(3): 340-347 |
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| Authors: | LI Yajun LI Ji'an ZHANG Jianbin GUO Ruiling LIN Huimin |
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| Affiliation: | (State Key Lab.of New Drug and Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,Shanghai 201203) |
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| Abstract: | The crude pingyangmycin(1)chelating copper was purified by column-based chromatography with the prepacked column of monodisperse polymer microspheres,and the HPLC purity of 1 chelating copper reached above 98%.The crude 1 chelating copper was purified sequencely by reversed column-based chromatography with the prepacked column of XR 3SP microspheres and POlyRP 30-300 microspheres.The purify and yield for the first step reached over 50%and 85%,while those for the second step were up to 98%and 88%.The total yield reached above 75%.The process was simple,reliable and suitable for industrial production. |
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| Keywords: | pingyangmycin chelating copper reverse phase chromatography separation and purification |
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