Addition of fish oil to atherogenic high fat diet inhibited atherogenesis while olive oil did not,in LDL receptor KO mice |
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| Affiliation: | 1. The Bert W. Strassburger Lipid Center, Sheba Medical Center, Tel-Hashomer, 5265601, Ramat Gan, Israel;2. Sackler Faculty of Medicine, Tel-Aviv University, Tel Aviv, Israel;3. Institute of Pathology, Sheba Medical Center, Tel-Hashomer, 5265601, Ramat-Gan, Israel;4. Achva Academic College, Israel;1. Center for Lipid Metabolomics, Division of Preventive Medicine, Brigham and Women''s Hospital, Harvard Medical School, Boston, MA, USA;2. Division of Preventive Medicine, Brigham and Women''s Hospital, Boston, MA, USA;3. Quest Diagnostics, San Juan Capistrano, CA, USA;4. VAP Diagnostics Laboratory, Birmingham, AL, USA;5. Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA;6. California Cardiovascular Institute, Fresno, CA, USA;7. Children''s Hospital Oakland Research Institute, Oakland, CA, USA;8. Division of Cardiovascular Medicine, Brigham and Women''s Hospital and Harvard Medical School, Boston, MA, USA;1. Institute of Endocrinology and Nutrition, University of Valladolid, Rio Hortega University Hospital, Valladolid, Spain;2. Marques of Valdecilla University Hospital, IFIMAV. RETICEF, University of Cantabria, Santander, Spain;3. Clinical Pharmacology Service, Rio Hortega University Hospital, Valladolid, Spain;1. Institute of Cardiovascular Sciences and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Peking University Health Science Center, Beijing 100191, China;2. Center for Molecular and Translational Medicine, Peking University Health Science Center, Beijing 100191, China;1. Institute of Nephrology, Zhong Da Hospital, Southeast University School of Medicine, Nanjing City, Jiangsu Province, China;2. Centre for Nephrology, University College London (UCL) Medical School, Royal Free Campus, UK |
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| Abstract: | Background and aimsMediterranean diet has been associated with decreased cardiovascular morbidity and mortality. Both fish and olive oil are key components of this diet. Therefore, we compared their effects on nonalcoholic fatty liver disease (NAFLD) and atherogenesis in a mouse model, fed a high fat diet.Methods and resultsForty nine, female LDL receptor knockout (LDLR KO) mice were allocated into 3 groups and fed an atherogenic high fat (HF) diet for 9 weeks. The HF group was fed a high fat diet alone. A HF + OO group was fed a HF diet with added olive oil (60 ml/kg feed), and the third group (HF + FO) was fed a HF diet with added fish oil (60 ml/kg feed).Both additions of fish and olive oil, significantly decreased plasma cholesterol elevation compared to HF diet. Nevertheless, only fish oil addition reduced significantly atherosclerotic lesion area by 51% compared to HF group. Liver levels of eicosapentenoic (EPA) and docosahexaenoic (DHA) acids were several folds higher in HF + FO group than in HF and HF + OO groups. Liver levels of oleic acid were higher in HF + OO compared to the other groups. Moreover, Fish oil addition significantly decreased NAFLD scores related to steatosis and inflammation and lowered the expression of the inflammatory genes interleukin 6 (IL6) and monocyte chemoattractant protein 1 (MCP1).ConclusionThese results suggest that fish oil addition on top of an atherogenic, HF diet, is beneficial, while olive oil is not, in its effect on plaque formation and NAFLD in LDLR KO mice. |
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| Keywords: | Atherosclerotic lesion Nonalcoholic fatty liver disease LDL receptor knockout mice Fish oil Olive oil High fat diet |
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