| 当归醇沉组分对肝CYP同工酶的影响 |
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| 引用本文： | 王智勇,张志善,等.当归醇沉组分对肝CYP同工酶的影响[J].现代中西医结合杂志,2001,10(11):1006-1007. |
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| 作者姓名： | 王智勇 张志善 |
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| 作者单位： | 王智勇（湖北医科大学药理教研室,武汉市,430071）
彭仁琇（湖北医科大学药理教研室,武汉市,430071）
张志善（湖北医科大学药理教研室,武汉市,430071） |
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| 摘 要： | 目的 :观察当归醇沉组分对肝 CYP同工酶的影响。方法 :采用 DEAE-纤维素作为分离递质 ,用 3种 p H值不同的洗脱液将当归醇沉物 (ESA)分离为 3种组分 :ESA-1、ESA-2、ESA-3。结果 :发现 ESA和 ESA-3可使小鼠肝脏微粒体中总 CYP量增加 ,ESA-2可抑制 7-乙氧异恶唑 -0 -脱乙基酶 (EROD)的活性 ,ESA-1、 ESA -2、 ESA-3均降低苯胺羟化酶 (AN H)的活性 ,ESA -2则升高红霉素 N-脱甲基酶 (ERD)的活性。结论 :当归可通过影响 CYP同工酶而发挥其在药物相互作用、抗肿瘤、抗氧化等方面的功能。
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| 关 键 词： | 当归醇沉物(ESA) DEAE-纤维素 分离 细胞色素P450 |
Effects of Chinese Angelica sinensis ethanol sediments on CYP isoenzyme |
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| Wang Zhiyong,Peng Renxiu,Zhang Zhishan.Effects of Chinese Angelica sinensis ethanol sediments on CYP isoenzyme[J].Modern Journal of Integrated Chinese Traditional and Western Medicine,2001,10(11):1006-1007. |
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| Authors: | Wang Zhiyong Peng Renxiu Zhang Zhishan |
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| Institution: | Wang Zhiyong,Peng Renxiu,Zhang Zhishan Department of Pharmacology,Hubei Medical University,Wuhan 430071 |
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| Abstract: | Objective:It is to study the effect of Chinese Angelica sinensis ethanol sediments on CYP isoenzyme.Methods:DEAE-Cellulose was used for isolation of ESA(ethanol sediments from Angelica Sinensis). Three components were obtained from ESA under three different pH chromatic fluid: ESA-1,ESA-2 and ESA-3. Results: ESA and ESA-3 can increase the content of CYP in mice liver. ESA-2 can inhibit the activity of EROD, but it can increase the activity of ERD. ESA-1, ESA-2 and ESA-3 all can inhibit the activity of ANH. Conclusion:Angelica sinensis functioned in the aspectis such as drug interaction, anti-tumor,anti-oxidation by influenced CYP isopenzyme. |
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| Keywords: | ESA(ethanol sediments from Angelica Sinensis) DEAE-Cellulose isolation cytochrome P450(CYP) |
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