异氟醚对新生大鼠认知功能的影响

目的 观察异氟醚对新生大鼠认知功能的影响。方法 新生1周Wistar大鼠40只，雌雄不拘，随机分为5组（n=8）：对照组（A组）吸入空气，B、C组分别吸入1．8％和1．2％异氟醚6h，D、E组分别吸入1．8％、1．2％异氟醚3次，1次／日，2h／次。吸入异氟醚后3周进行认知功能测试，包括旷场实验和Morris水迷宫测试，认知功能测试完毕后立即处死大鼠，取海马组织，进行免疫组织化学染色，测定N-甲基-D-天冬氨酸受体-2（NMDAR2）及谷氨酸转运体（GLAST）的表达。结果 吸入异氟醚可导致大鼠认知功能降低，海马NMDAR2和GLAST表达增加，吸入高浓度异氟醚后海马NMDAR2和GLAST表达增加更明显，单次吸入和多次吸入异氟醚后海马NMDAR2和GLAST表达差异无统计学意义（P〉0．05）。结论 异氟醚可降低新生大鼠认知功能，与上调海马NMDAR2及GLAST表达有关。

Effects of isoflurane anesthesia on cognitive function in neonatal rats

Objective To investigate the long-term changes in cognitive function and glutamatergic N-methyl-D-aspartate receptor-2 (NMDAR_2) and GLAST expression in hippocampus after isoflurane anesthesia in neonatal rats.Methods Forty 1 week old Wistar rats of either sex were randomly divided into 5 groups (n=8, each):group A control,group B and C inhaled 1.8% and 1.2% isoflurane for 6 h respectively;group D and E inhaled 1.8% and 1.2% isoflurane 2 h per day for 3 days respectively.Cognitive function was assessed using open fieled and Morris water maze test at 21 d after last anesthesia.The animals were killed immediately after the tests and hippoeampus was isolated for determination of NMDAR_2 and GLAST expression using immuno-histochemical technique.Results Isoflurane anesthesia induced cognitive decline and increased NMDAR_2 and GLAST expression in hippocampus dose-dependently.But there was no significant difference in NMDAR_2 and GLAST expression between group B and D and group C and E.Conclusion Isoflurane anesthesia can induce long-lasting cognitive decline and increase the expression of glutamatergic NMDAR_2 and GLAST in hippoeampus in neonatal rats.