气相色谱法同时测定无极膏中5个挥发性有效成分的含量

目的:建立同时测定无极膏中合成樟脑、薄荷脑、水杨酸甲酯、冰片和麝香草酚5种主要成分含量的分析方法。方法:采用气相色谱法,色谱柱为聚乙二醇(PEG)-20M毛细管色谱柱(30 m×0.32 mm×0.25μm),检测器为FID检测器,进样口温度为250℃,检测器温度为250℃。升温程序为初始温度150℃,保持5 min;10℃.min-1升温至200℃,保持8 min;再以20℃.min-1升温至240℃,保持25 min,分流比为10∶1。结果:合成樟脑、薄荷脑、水杨酸甲酯、冰片和麝香草酚浓度分别在1.4934～7.4672 mg.mL-1(r=0.9999),0.9304～4.6520 mg.mL-1(r=0.9999),0.9075～4.5376 mg.mL-1(r=0.9999),0.1387～0.6936 mg.mL-1(r=0.9999),0.0716～0.3580 mg.mL-1(r=0.9999)质量浓度范围内线性关系良好。平均回收率(n=9)分别为98.8%,99.4%,98.6%,99.6%,99.7%。结论:该方法灵敏、快速简便、准确,可有效地控制无极膏的质量。

GC simultaneous determination of fivev olatile effective components in Compositus Mentholi cream

Objective:To develop a GC method for the determination of the five effective components in Compositus Mentholi cream.Methods:The GC was performed on a PEG-20M capillary column(30 m×0.32 mm×0.25 μm)with an FID detector.The injector temperature was 250 ℃ and detector temperature was set at 250 ℃.The temperature program of column oven was set as follows:150 ℃ for 5 min,increased to 200 ℃ at a rate of 10 ℃·min-1 and kept for 8 min,then at 20 ℃·min-1 to 240 ℃,held for 25 min.The injection split ratio was 10∶ 1.Results:The calibration curves of synthetic camphor,menthol,methyl salicylate,synthetic borneol and thymol were linear in the concentration range of 1.4934-7.4672 mg·mL-1(r=0.9999),0.9304-4.6520 mg·mL-1(r=0.9999),0.9075-4.5376 mg·mL-1(r=0.9999),0.1387-0.6936 mg·mL-1(r=0.9999),0.0716-0.3580 mg·mL-1(r=0.9999),respectively.The mean recoveries(n=9)were 98.8%,99.4%,98.6%,99.6%,99.7%,respectively.Conclusion:The method is simple,rapid,accurate and reproducible.It can be used as the determination control method for Compositus Mentholi cream.