HPLC法测定金菊五花茶颗粒中芦丁和蒙花苷的含量

目的 建立金菊五花茶颗粒中芦丁和蒙花苷的含量测定方法.方法 采用高效液相色谱法,色谱柱Eclipse XDB-C18柱（4.6 mm×150 mm,5μm）,流动相为甲醇（A）-乙腈（B）-0.1％磷酸溶液（C）,梯度洗脱,流速1.0mL·min^-1;检测波长334 nm；柱温30℃.结果 芦丁线性范围在21.22～339.58μg,r=0.999 4,样品加样平均回收率为100.0％（RSD=1.10％）；蒙花苷线性范围在1.75 ～ 28.08μg,r =0.999 3,样品加样平均回收率为99.2％（RSD=1.17％）.结论 该方法简便、准确,可用于制剂的质量控制.

Determination of rutin and linarin in Jinju Wuhuacha Granules by HPLC

Objective To establish a method for determining the contents of rutin and linarin in Jinju Wuhuacha Gran- ules by HPLC Methods An Eclipse XDB - C18 column（4.6 mm × 150 mm,5 μm） was used with methanol（A） - aceto- nitrile（B） - 0.1% phosphoric acid（C） as the mobile phase The flow rate was 1.0 mL · min ^-1, and the UV - detection wavelength was set at 334 nm,column temperature st 30 ℃ Results The calibration craves for turin between 21.22 and 339.58 μg（r=0.999 4） and for linarin between 1.75 and 28.08 μg（r=0.999 3） were linear Recoveries were 100.0% with RSD 1.10% for rutin and 99.2% with RSD 1.17% for linarin. Conclusion The lnethod was silnple and reliable for the quality control of Jinjuwuhuaeha Granules.