ERK1/2信号通路介导丙泊酚对 H2O2诱发的心肌细胞损伤保护作用 |
| |
引用本文: | 梁艳丽,唐敏,徐聪敏.ERK1/2信号通路介导丙泊酚对 H2O2诱发的心肌细胞损伤保护作用[J].山东医药工业,2014(5):257-260. |
| |
作者姓名: | 梁艳丽 唐敏 徐聪敏 |
| |
作者单位: | 广东湛江中心人民医院麻醉科,广东湛江524037 |
| |
摘 要: | 目的:研究丙泊酚对 H2 O2诱发的心肌细胞损伤的保护作用及其作用机制。方法采用胰酶消化法获取大鼠胎鼠心肌细胞,以 H2 O2损伤心肌细胞获得心肌缺血再灌注损伤实验模型;加入不同浓度丙泊酚(12.5、25、50μmol·L -1),MTT 法检测细胞存活率;用硫代巴比妥酸法、黄嘌呤氧化酶法分别测定各组细胞培养液中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性;流式细胞术检测细胞凋亡水平;Western blotting 检测细胞中 ERK1/2、Bcl -2及 Bax 的表达。结果丙泊酚(12.5、25、50μmol·L -1)能抑制由 H2 O2导致的细胞死亡(P 〈0.01);减少MDA 的产生(P 〈0.01),提高 SOD 活性(P 〈0.01);25、50μmol·L -1丙泊酚能抑制由 H2 O2导致的细胞凋亡(P 〈0.05);25μmol·L -1丙泊酚作用于细胞后能激活 ERK1/2磷酸化,增加 Bcl -2且减少 Bax 的表达。结论丙泊酚通过激活 ERK1/2磷酸化对 H2 O2诱发的心肌细胞损伤起到保护作用。
|
关 键 词: | 丙泊酚 心肌细胞 ERK1 2 过氧化氢 |
ERK1/2 signal pathWay mediated propofol protected on cultured rat cardiomyocytes damaged by H2 O2 |
| |
Authors: | LIANG Yan-li TANG Min XU Cong-min |
| |
Institution: | ( Anesthesia Department, Central People's Hospital of Zhanjiang, Zhanfiang 524037, China ) |
| |
Abstract: | Objective To investigate the influence and mechanism of propofol on cardiomyocytes damaged by H2 O2 . Methods Cardiomyocytes from the hearts of 1 - 3 - day - old neonatal rats were prepared by a modified method. Treated cultured rat cardiomyocytes by H2 O2 ,propofol(12. 5,25,50 μmol·L - 1 ). MTT was used to detect cell viability. Malondial-dehyde(MDA)was determined by measuring thiobarbituric acid - reactive substances. Superoxide dismutase(SOD)was assayed by xanthine oxidase method. The rates of apoptosis of cardiomyocyte were detected by flow cytometry. The expression of ERK1 / 2,Bcl - 2 and Bax were tested by western blotting. Results Propofol suppressed the cardiomyocyte death induce by H2 O2(P 〈 0. 01),and decreased the production of MDA(P 〈 0. 01),promoted the ability of SOD(P 〈 0. 01). The ap-optosis rates of propofol group significant different from that of H2 O2 group(P 〈 0. 05). Propofol activated the phosphorated ERK1 / 2. The expression of Bcl - 2 was increased while that of Bax was suppressed. Conclusion Propofol protected on cul-tured rat cardiomyocytes damaged by H2 O2 via ERK1 / 2 signal pathway. |
| |
Keywords: | Propofol Cardiomyocytes ERK1/2 H2 O2 |
本文献已被 维普 等数据库收录! |