| HPLC法测定依诺沙星胶囊的溶出度 |
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| 引用本文： | 蔡群,孔令洋,孙巧巧.HPLC法测定依诺沙星胶囊的溶出度[J].山东医药工业,2014(2):96-97. |
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| 作者姓名： | 蔡群 孔令洋 孙巧巧 |
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| 作者单位： | [1]济宁市第一人民医院,山东济宁272100 [2]济宁市药品检验所,山东济宁272100 |
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| 摘 要： | 目的 建立依诺沙星胶囊的溶出度测定方法.方法 采用十八烷基硅烷键合硅胶为填充剂,以0.025mol·L^-1磷酸溶液（用三乙胺调节pH至3.0）-甲醇-乙腈（80：10：10）为流动相,检测波长为269 nm;分别采用HPLC法和药典规定的紫外对照品对照法测定依诺沙星胶囊的溶出度.结果 依诺沙星在5～ 200 μg·mL^-1范围内线性良好（r =0.999 9,n=6）,回收率为100.6％,RSD=0.6％.结论 本方法准确、灵敏、重现性好、可行.
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| 关 键 词： | 溶出度 依诺沙星胶囊 紫外分光光度法 高效液相色谱法 |
Determination of the dissolution of Enoxacin Capsules by HPLC |
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| Authors: | CAI Qun KONG Ling-yang SUN Qiao-qiao |
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| Institution: | 1. Jining First People＇s Hospital,Jining 272100,China;2. Jining Institute for Drug Control,Jining 272100,China) |
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| Abstract: | Objective To establish the assay method of dissolution test on Enoxacin Capsules. Methods A Phe- nomenex C18 Column was used with the mobile phase of 0. 025 mol·L^-1 phosphoric acid （ pH was adjusted to 3 with trieth- ylalnine ） - lnethanol - acetonitrile （ 80：10：10）. The determination wavelength was 269 nm. The method of HPLC and the method of UV was compared. Results The Enoxacin Capsules was linear within the concentration range of 5 ～ 200μg · mL ^-1（r=0. 999 9,n=6）. The average recovery was 100.6% and RSD was 0.6%. Conclusion The result shows that the method was accurat, reliable, recurrent well, and can be used for the quality control. |
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| Keywords: | Dissolution Enoxacin Capsules UV HPLC |
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