3种血清学抗体检测在类风湿关节炎诊断中的应用

目的分析类风湿因子（RF）、抗环瓜氨酸肽抗体（抗-CCP）和抗-RA33 3种血清指标在类风湿关节炎（RA）诊断中的敏感性和特异性,探讨其对RA的临床诊断价值。方法 RA患者78例、非RA患者52例和健康对照组80例血清分别采用酶联免疫吸附试验检测抗-CCP和抗-RA33、速率散射比浊法检测RF,观察各指标对RA诊断价值。结果 3种血清学指标对RA诊断的敏感性由高到低依次是RF、抗-CCP、抗-RA33,特异性由高到低依次为抗-RA33、抗-CCP、RF。结论抗-CCP与RF联合检测对筛查和辅助诊断RA具有较好的临床应用价值。

Diagnostic value of three auto-antibodies in patients with rheumatoid arthritis

Objective To investigate the level of three auto-antibodies including RF,CCP and RA33 in serum of rheumatoid arthritis（RA） patients and their clinical diagnostic value in RA.Methods The serum samples of 78 patients with RA,52 non-RA rheumatic patients and 80 healthy volunteers were collected.The level of RF was measured by the rate scatting immunity methods.The expression of anti-CCP and anti-RA33 were measured by ELISA methods.Results The positive rates of all three auto-antibodies in RA patients were significantly higher than those of other group（P0.05）.The sensitivity of combined detection to RA was significantly decreased while the specificity was highly increased to 97.5%.Conclusion The combination of RF and anti-CCP could improve the specificity and sensitivity which may be helpful for clinical diagnosis.