基因芯片技术分析与冠心病的相关基因

目的评估血管紧张素原(angiotensinogen,AGT)、内皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)和载脂蛋白E(apolipoprotein E,ApoE)多态性与冠心病的关系,并分析AGT、eNOS和ApoE基因多态性在冠心病的发生和发展中是否存在协同作用。方法选择冠心病患者133例及对照者154名,用基因芯片技术检测AGT、eNOS和ApoE基因多态性,并对比其基因型及等位基因频率。结果冠心病组AGT TT和eNOS TT基因型频率与对照组相比差异有统计学意义,分别为OR=2．63,P<0．001,OR=8．50,P< 0．05。AGT和eNOS基因多态性与冠心病相关。同时携带AGT TT和eNOS TT基因型者与冠心病密切相关(OR=1．05,P<0．01)。ApoE基因型频率及等位基因频率与对照组相比差异无统计学意义(P>0．05)。结论AGT和eNOS基因多态性可能是中国人冠心病的危险因素,基因芯片技术为研究多种易感基因与冠心病的相关性提供了一项高效、敏感的方法。

An analysis of gene polymorphisms in predisposition to coronary disease with gene chip technology

Objectives To evaluate the involvement of the angiotensinogen (AGT) gene polymorphism, endolhelial nitric oxide synthase (eNOS) gene polymorphism and apolipoprotein E (ApoE)gene polymorphism in predisposition to coronary disease (CHD), as well as their possible synergetic effect in the development of CHD. Methods The study included 287 subjects (133 with CHD and 154 controls). Gene chip technology was performed. The allele frequency and genotype distribution were compared between the two groups. Results Significantly higher frequencies of the TT genotypes of AGT and eNOS gene were observed in CHD group. The AGT and eNOS gene polymorphisms were associated with CHD (OR=2.63, P<0.001, OR=8.50 ,P<0.05, respectively). Subjects carrying AGT TT and eNOS TT genotype showed a stronger association with CHD (OR=1.50, P<0.01). No significant difference was observed in ApoE allele frequency and genotype distribution between the two groups (P>0.05). Conclusions The AGT and eNOS gene polymorphisms may be risk factors of CHD. Gene chip technology provides an effective and sensitive method to study the relationship of gene polymorphisms and CHD.