| 体外受精牛胚胎切割后的超低温冷冻研究 |
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| 引用本文： | 上官步敏,阳年生,李树静,余文莉. 体外受精牛胚胎切割后的超低温冷冻研究[J]. 国外畜牧学(草食家畜), 2005, 0(2): 40-42 |
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| 作者姓名： | 上官步敏 阳年生 李树静 余文莉 |
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| 作者单位： | 加拿大太平洋遗传中心,abbostsford B.C.Canada V2S 5Z5;北京安伯胚胎生物技术中心,北京,100081 |
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| 摘 要： | 本文研究了不同冷冻方法和不同冷冻保护剂对牛7日和8日龄IVF切割胚胎冷冻效果的影响。试验结果表明：在乙二醇冷冻液中添加一定浓度的透明质酸不能改善胚胎的抗冻能力；用细管法玻璃化冷冻和两步解冻法可显著提高体外受精胚胎的存活和生长。
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| 关 键 词： | 体外受精 胚胎切割 超低温冷冻 透明质酸 细管法玻璃化冷冻 两步解冻法 |
| 文章编号： | 1003-6377(2005)02-0040-03 |
| 修稿时间： | 2005-04-12 |
Cryopreservation of Biopsied Bovine IVF Blastocysys |
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| Rich Vanderwal,Marty Darrow,SHANGUAN Bu-yun,YANG Nian-sheng,Rich Vanderwal,Marty Darrow,LI Shu-jing,YU Wen-li. Cryopreservation of Biopsied Bovine IVF Blastocysys[J]. Grass-Feeding Livestock, 2005, 0(2): 40-42 |
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| Authors: | Rich Vanderwal Marty Darrow SHANGUAN Bu-yun YANG Nian-sheng Rich Vanderwal Marty Darrow LI Shu-jing YU Wen-li |
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| Abstract: | Different cryoprotective methods and cryotor were used to study the influence of day-7 and day-8 biopsied bovine IVF blastocysts. The results showed that there was no improvement to add hyaluronic acid to glycol.The rates of survival and usability of vitrified by straw method and two-step thawing method could be improved significantly. |
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| Keywords: | in vitro fertilization biopsy cryopreservation hyaluronic acid straw two-step thawing |
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