钛表面粗糙度对牙周韧带细胞早期附着影响的荧光观察

背景:种植体表面粗糙程度对组织细胞的增殖、分化及基因表达有直接影响.目的:观察牙周韧带细胞在不同纯钛表面粗糙度的早期附状态,以及材料表面性能对组织细胞分化的影响.设计、时间及地点:随机对照观察,多样本比较实验,于2005-01/2006-07在赣南医学院科研中心完成.材料:采用机床切割机将商业纯钛棒制备成直径为10mm厚2mm的钛片,共24个,分为4组:机械处理组,硝酸处理组,喷砂处理组,综合处理组,每组6个样品.方法:采用TR240便携式表面粗糙度仪测定表面粗糙度.机械处理组:进行单纯的机械处理.硝酸处理组:单纯的机械处理+65%的硝酸(100℃,1 h)处理.喷砂处理组:单纯的机械处理+100 μ m的Al2O3喷砂处理.综合处理组:单纯的机械处理+100 μ m的Al2O3喷砂处理后+65%的硝酸(100℃,1 h)处理.主要观察指标:样品在DMEM中预置30 m后接种第3代的细胞,分别于30,60,120,240 min,1,3,7 d取出钛片,于荧光显微镜下观察各组纯钛表面的粗糙度和牙周韧带细胞在不同表面粗糙度的纯钛表面早期附着情况.结果:①定量分析:4组粗糙度分别为:机械处理组(599.5±8.3)nm、硝酸处理组(406.5±4.6)nm、喷砂处理组(358.8±11.8)nm、综合处理组(8.7±2.0)nm,机械处理组高于硝酸处理组、喷砂处理组和综合处理组(P<0.01),差异有显著性意义(P<0.01).②定性分析:牙周韧带细胞在纯钛表面早期附着的荧光观察显示,随着时间的推移,各组早期附着在纯钛表面的牙周韧带细胞增多,细胞增殖良好.但粗糙度大的钛表面,细胞附着较少,粗糙度小的钛表面,细胞附着较致密些.结论:钛表面粗糙度越小,牙周韧带细胞早期附着越多,有利于细胞黏附和增殖.

Influence of surface roughness of titanium on the early attachment of human periodontal ligament cells A fluorescent study

BACKGROUND: Surface roughness of implants can directly influence cellular proliferation, differentiation, and gene expression. OBJECTIVE: To observe the early attachment of periodontal ligament cells (PDLCs) to pure titanium with different surface roughness levels, and to study the effect of surface performance on cell differentiation. DESIGN, TIME AND SETTING: Randomized controlled observation/multi-sample comparison study, which was performed at Center of Science and Technology, Gannan Medical College between January 2005 and July 2006. MATERIALS: Pure titanium stick was cut into pieces, of 10 mm diameter and 2 mm thickness, using cutting-off machine, and there were 24 sections in total. Then, the titanium sections were randomly divided into four groups: simple mechanical processing, nitric acid processing, sand blasting processing, and combination group, with 6 sections per group. METHODS: TR240 portable-type surface roughness meter was used in this study. In the simple mechanical processing group, sections were scoured by sand paper alone; in the nitric acid processing group, sections were etched with 65% HNO3 for 1 hour at 100 ℃ after scoured by sand paper; in the sand blasting processing group, sections were sandblasted by 100 μ m AI203 after scoured by sand paper; in the combination group, sections were etched with 65% HNO3 for I hour at 100～C after scoured by sand paper and sandblasted by 100 μ m Al2O3. MAIN OUTCOME MEASURES: Samples were maintained in DMEM for 30 minutes, and the third-passage cells were inoculated. Then, titanium sections were taken out at different time points of 30, 60, 120, 240 minutes, 1, 3, and 7 days. Surface roughness and early attachment of PDLCs were detected under fluorescent microscope. RESULTS: O Quantitative analysis: Surface roughness was (599.5±8.3) nm in the simple mechanical processing group, (406.5 +4.6) nm in the nitric acid processing group, (358.8±11.8) nm in the sand blasting processing group, and (8.7±2.0) nm in the combination group. On the other hand, surface roughness in the simple mechanical processing group was significantly higher Fluorescence observation exhibited that number of PDLCs attaching to pure titanium surface was increased, and the proliferation was greater with the time passing by. in addition, surface roughness of pure titanium was positively associated with number of PDLCs. CONCLUSION: The lighter the surface roughness is, the more the early attachment of PDLCs is, benefiting for cell adhesion and proliferation.