| 化淤理肺方对肺纤维化大鼠MMP 2和TIMP 1表达的影响 |
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| 引用本文： | 王琴,刘世青,李长生,鹿伟
.化淤理肺方对肺纤维化大鼠MMP 2和TIMP 1表达的影响[J].山东大学学报(医学版),2009,47(2):14-17. |
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| 作者姓名： | 王琴 刘世青 李长生 鹿伟
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| 作者单位： | 王琴,WANG Qin(山东大学医学院,济南,250012);刘世青,LIU Shi-qing(山东大学附属千佛山医院呼吸科,济南,250014);李长生,LI Chang-sheng(山东大学附属千佛山医院中医科,济南,250014);鹿伟,LU Wei(山东大学附属千佛山医院病理科,济南,250014)
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| 摘 要： | 目的探讨化淤理肺方对肺纤维化的干预作用和对MMP 2/TIMP 1表达的影响。方法肺纤维化Wistar雄性大鼠84只,随机分成4组:中药组、激素组、模型组和对照组。所有大鼠第7、14、28天各处死7只。经HE染色评价肺组织肺泡炎和肺纤维化程度，免疫组化检测法检测MMP 2与TIMP 1的表达。结果① 肺泡炎、肺纤维化程度评价：模型组肺泡炎和肺纤维化程度较重,且胶原纤维沉积较多;中药组可见炎性细胞较少,肺泡结构基本正常,肺泡炎与肺纤维化较轻;激素组肺泡炎及纤维化与中药组相似。 ② MMP 2蛋白的表达：中药组第7、14、28天较模型组表达明显减轻，于第14天达到高峰，此后降低，表达水平与激素组相似。③ TIMP 1蛋白表达：中药组在这3个时段的表达均较对照组增高, 但均较模型组低，且呈逐渐升高趋势。结论化淤理肺方能减轻博来霉素诱导的大鼠肺纤维化,可能是通过调节MMP 2、TIMP 1途径起作用。
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| 关 键 词： | 肺纤维化 化瘀理肺方 基质金属蛋白酶 2 基质金属蛋白酶组织抑制物 大鼠 Wistar |
Effects of Huayulifei on the expressions of MMP-2 and TIMP-1 in rats with bleomycin-induced pulmonary fibrosis |
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| WANG Qin,LIU Shi-qing,LI Chang-sheng,LU Wei.Effects of Huayulifei on the expressions of MMP-2 and TIMP-1 in rats with bleomycin-induced pulmonary fibrosis[J].Journal of Shandong University:Health Sciences,2009,47(2):14-17. |
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| Authors: | WANG Qin LIU Shi-qing LI Chang-sheng LU Wei |
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| Institution: | 1.School of Medicine;Shandong University;Jinan 250012;China;2.Department of Respiratory;3.Department of Traditional Chinese Medicine;4.Department of Pathology;Qianfoshan Hospital;Jinan 250014;China |
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| Abstract: | Objective To evaluate the effects and mechanism of Huayulifei on blemycin-induced pulmonary interstitial fibrosis in rats.Methods Rats were randomly divided into the control group,the model group,the Huayulifei group and the hydrocortisone group.Rat pulmonary fibrosis was induced by blemycin(5 mg/kg) in the experimental groups and 0.9% saline in the control group.Rats were killed on the 7th,14th and 28th days.Expressions of MMP-2 and TIMP-1 proteins were determined by an immunohistochemical technique.Result... |
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| Keywords: | Pulmonary fibrosis Huayulifei Matrix metallopeptidase-2 Tissue inhibitor of metalloproteinase-1 Rats Wistar |
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