单眼视觉剥夺鸽不同脑区tubulin和chs rex b 同源基因的差异表达

目的：检测tubulin与chs rex b同源基因在左眼视损毁后鸽脑不同脑区的差异表达，方法：以经消减抑制杂交（suppression subtraction hybridization SSH)方法获得的鸽脑差异表达基因的表达序列标签（expressed sequence tag,EST)为探针，用放射性同位素对其进行标记，藉Northern杂交法分析其mRNA在不同脑区的丰度，结果；与人tubulin有高度同源性及与鸡的chs-rex-b m,RNA有高度同源性的基因 左，右视盖的丰度明显高于左，右前脑，结论：tubulin与rex b同源基因在鸽脑视觉相关脑区的发育过程中可能具有某此独特作用。

Expressions of Tubulin andChs Rex b Homologous Gene in Different Brain Regions of Monocular Eye Deprived Pigeon

Objective To observe the expression of tubulin and chs rex b homologous gene in different region of monocular eye deprived pigeon.Methods The differential expressed gene in the pigeon brain was used as probe and labeled with radioisotope.The expressions of both genes were analyzed by Northern hybridization.Results The expressions of tubulin and chs rex b homologous genes are higher in optic tecta than in forebrain, and they are higher in left optic tecta than in right one.Conclusion Tubulin and chs rex b homologous genes in the brain of pigeon obtained by SSH have relations with the molecular construction of vision pathway.