Dual effect of melatonin as proinflammatory and antioxidant in collagen-induced arthritis in rats |
| |
Authors: | Jiménez-Caliani Antonio J Jiménez-Jorge Silvia Molinero Patrocinio Guerrero Juan M Fernández-Santos Jose M Martín-Lacave Inés Osuna Carmen |
| |
Institution: | Departments of Medical Biochemistry and Molecular Biology;, and Normal and Pathologic Citology and Histology, The University of Seville School of Medicine and Virgen Macarena Hospital, Seville, Spain |
| |
Abstract: | Abstract: The aim of this study was to determine the effects of melatonin on proinflammatory status of rats with collagen-induced arthritis (CIA). CIA was induced in male Wistar rats with an emulsion of type II collagen in Freund's Incomplete Adjuvant (C-II/FIA). For 14 days, control and pinealectomized rats received a subcutaneous injection of 100 μ L melatonin (30 μ g) or vehicle (saline on 1% ethanol). Levels of cytokines interleukin (IL)-1 β and IL-6 were determined in the serum, peripheral blood mononuclear cells, and joints. Levels of anti-type II collagen antibody, nitrite/nitrate, and lipid peroxidation (LPO) were determined in the serum, joints, and brain. Treatment with melatonin significantly increased the levels of IL-1 β , IL-6, nitrite/nitrate and LPO in joints. However, melatonin significantly reduced the levels of nitrite/nitrate and LPO in serum and brain. Moreover, CIA in pinealectomized rats presented significantly reduced levels of IL-1 β and IL-6, titers of anti-type II collagen antibodies, levels of nitrite/nitrate, and LPO in joints but elevated levels in serum and brain. Melatonin has been described as a proinflammatory and antioxidant agent. In a process of inflammation as CIA, melatonin acts with a markedly proinflammatory effect at local and peripheral levels maintaining its antioxidant effect only at peripheral level. |
| |
Keywords: | collagen-induced arthritis inflammation interleukins melatonin oxidative stress |
本文献已被 PubMed 等数据库收录! |
|