汉防己碱治疗重症急性胰腺炎的实验研究

目的研究汉防己碱（Tet）用于治疗重症急性胰腺炎（SAP）作用及其机制。方法将45只SD大鼠随机分为对照组、重症急性胰腺炎组（SAP组）及汉防已碱组（Tet组）。各组术后3h、6h、12h随机取5只大鼠，开腹取材检测。检测血钙离子浓度，胰腺腺泡内钙离子荧光强度及胰腺病理评分。结果对照组检测指标在组内各时间段数值比较无统计学意义（P〉0．05）。SAP组和Tet组检测在组内各时间段及组间比较均有统计学意义（P〈0．05）。结论本实验证实Tet作为钙离子的阻滞剂可明显抑制钙离子进入胰腺腺泡细胞，有效减轻实验大鼠胰腺的病理损害；钙超载在SAP发展中起重要作用。

Tetrandrine treatment of severe type acute pancreatitis

Objective To study the effect and mechanism of tetrandrine (Tet) treatment of severe acute pancreatitis (SAP). Methods 45 SD rats were randomly divided into 3 groups: control group, severe acute pancreatitis group (SAP group) and tetrandrine group (Tet group). 5 rats were randomly taken from each group 3, 6, and 12 h after operation, and then underwent laparotomy for testing. Detection targets included calciumion concentration, pancreatic acinar calcium fluorescence intensity and pancreas pathological evaluation. Results In terms of the value of all detection targets, there was no statistical difference for the control group between different time points (P ＞0.05 )while there was statistical difference for SAP group and TET group in different time points (P＜0.05). Conclusion The experiment confirms that Tet, as a calcium blocker, can inhibit calcium to penetrate into pancreatic acinar cells, thus effectively reduce the pathological damage to pancreas of the experimental rats. Calcium overload plays an important role in SAP development.