复方银杏叶胶囊对肝损伤大鼠CYP2E1、CYP3A4的影响

目的研究复方银杏叶胶囊(CGB)对酒精性肝损伤大鼠CYP2E1、CYP3A4活性的影响。方法正常组和酒精性肝损伤模型组均以CGB[(250 mg/(kg.d)]灌胃,分别在灌胃CGB前及灌胃1周后,灌胃探针药氯唑沙宗(50 mg/kg)及氨苯砜(20 mg/kg),于探针药灌后24 h内不同时间点采血,测定各探针药血药浓度。结果灌胃CGB前,模型组氯唑沙宗和氨苯砜的AUC0-24、Cmax均显著低于正常组(P<0.05或P<0.01)。灌胃CGB后,模型组氯唑沙宗和氨苯砜的AUC0-24、Cmax均较灌胃前显著升高(P<0.05或P<0.01);且氯唑沙宗的t1/2灌胃CGB后明显高于灌胃前(P<0.05)。结论 CGB能够明显抑制酒精性肝损伤大鼠CYP2E1、CYP3A4酶活性。

Influence of the compound Ginkgo Biloba on CYP2E1 and CYP3A4 enzyme activity in hepatic injuried rats

Objective To observe the effect of the compound Ginkgo Biloba（CGB） on CYP2E1 and CYP3A4 activity in hepatic alcohol-injuried rats.Methods CGB was orally administrated to the normal and the alcohol-injured hepatic injury model rats.Two group rats underwent 2-cycle pharmacokinetic experiments before and after being treated with CGB（250 mg·kg^-1·d^-1） for 7 days,in which the rats were concomitantly administered Chlorzoxazone（50 mg/kg） and Dapsone（20 mg/kg） by gastrogavage followed by blood-withdrawing from orbital bleeding at different intervals within 24 hours.Results Before being treated with CGB,compared with normal control group,the AUC0-24 and Cmax of Chlorzoxazone and Dapsone in the model group were decreased significantly（P〈0.05,P〈0.01）.However,the AUC0-24,and Cmax of Chlorzoxazone and Dapsone after being treated with CGB in the model group were both larger than those before treatment（P〈0.05,P〈0.01）,and the t1/2 of Chlorzoxazon than that that before treatment.Conclusion CGB could inhibit activities of CYP2E1 and CYP3A4 in alcohol-injuried rats.