| RGDS肽对离体大鼠血小板聚集和ERK1/2磷酸化的影响 |
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| 引用本文： | 张立克 刘玉玲 芦玲巧 姚兴海. RGDS肽对离体大鼠血小板聚集和ERK1/2磷酸化的影响[J]. 首都医科大学学报, 2005, 26(3): 291-292 |
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| 作者姓名： | 张立克 刘玉玲 芦玲巧 姚兴海 |
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| 作者单位： | 首都医科大学病理生理学教研室(张立克，刘玉玲，芦玲巧)，首都医科大学病理生理学教研室(姚兴海) |
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| 基金项目： | 北京市教委科技发展计划(99KJ15)资助项目 |
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| 摘 要： | 目的观察外源性精-甘-天-丝冬氨酸(RGDS)肽对离体大鼠血小板聚集及细胞外信号调节激酶1/2(ERK1/2)磷酸化的影响,探讨RGDS肽对血小板聚集的作用与ERK1/2磷酸化表达改变的关系。方法用比浊法测定血小板最大聚集率;Western-blot测定血小板ERK1/2磷酸化表达。结果RGDS肽抑制血小板聚集后伴随ERK1/2磷酸化表达减少。结论ERK1/2通路参与血小板聚集;抑制ERK1/2磷酸化表达可能是外源性RGDS肽抗血栓的机制之一。
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| 关 键 词： | 细胞外信号调节激酶1/2 血小板聚集 精-甘-天冬-丝氨酸肽 信号转导 |
| 收稿时间： | 2004-09-28 |
| 修稿时间： | 2004-09-28 |
Effects of RGDS Peptide on Aggregation and Phosphorylation of ERK1/2 in Rat Platelets in Vitro |
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| Zhang Like,Liu Yuling,Lu Lingqiao,Yao Xinghai. Effects of RGDS Peptide on Aggregation and Phosphorylation of ERK1/2 in Rat Platelets in Vitro[J]. Journal of Capital Medical University, 2005, 26(3): 291-292 |
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| Authors: | Zhang Like Liu Yuling Lu Lingqiao Yao Xinghai |
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| Affiliation: | Department of Pathophysiology, Capital University of Medical Sciences |
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| Abstract: | Objective To obsere influence of RGDS peptide on rat platelet aggregation rate and the ERK1/2 phosphorylation induced by different agonists in vivo and study the relationship between the change of ERK1/2 phosphorylation and the change of platelets aggregation with RGDS peptide. Methods Maximal aggregation rate(MAR) was measured with nephelometry; ERK1/2 phosphorylation was detected by Western blot. Results The MAR and phosphorylation of ERK1/2 were inhibited by RGDS peptide. Conclusion The phosphorylation of ERK1/2 was one of the cellular signal transduction mechanisms of the platelets aggregation; and the inhibition of ERK1/2 phosphorylation of may be one of the mechanisms that RGDS peptide antagonize thrombus. |
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| Keywords: | ERK1/2 platelets aggregation RGDS peptide cellular signal transduction |
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