Chitosan‐Coated Superparamagnetic Iron Oxide Nanoparticles for Doxorubicin Delivery: Synthesis and Anticancer Effect Against Human Ovarian Cancer Cells |
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| Authors: | Amaneh Javid Shahin Ahmadian Ali A Saboury Seyed M Kalantar Saeed Rezaei‐Zarchi |
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| Institution: | 1. Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, , Tehran, Iran;2. Center of Excellence of Nano‐Biomedicine, Nano‐Science and Nano‐Technology Research Center, University of Tehran, , Tehran, Iran;3. Research and Clinical Centre for Infertility, Shahid Sadoughi University of Medical Sciences, , Yazd, Iran;4. Department of Biology, Payame Noor University, , Yazd, Iran |
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| Abstract: | Doxorubicin‐loaded chitosan‐coated superparamagnetic iron oxide nanoparticles (Fe3O4; SPIO‐NPs) were prepared by coprecipitation and emulsification cross‐linking method and uniform NPs with an average particle size of 82 nm, with high encapsulation efficiencies, were obtained. The drug‐loading efficiency of doxorubicin (3.2 mg/mg NPs) showed better results for the chitosan‐loaded SPIO‐NPs as compared to the bare ones (0.5 mg/mg; p < 0.05). The incubation of A2780 and OVCAR‐3 human ovarian cancer cells with doxorubicin‐loaded and doxorubicin‐loaded chitosan‐coated SPIO‐NPs, for 24, 48, 72, 96, and 120 h, showed significant IC50 (2.0 ± 0.6 and 7.1 ± 2.7 mm doxorubicin) and IC90 (4.0 ± 9.2 and 10 ± 0.5 mm doxorubicin), respectively, after 96 h of incubation. While, 95% and 98% growth inhibition was seen in A2780 and OVCAR‐3 cells after the 96‐h exposure to the doxorubicin‐chitosan‐SPIO‐NPs (p < 0.05). A 5‐day (120 h) incubation with doxorubicin‐chitosan‐SPIO‐NPs showed that A2780 and OVCAR‐3 cells were able to uptake 120 and 110 pg iron/cell, respectively, when treated with doxorubicin‐chitosan‐SPIO‐NPs for 72 h (p < 0.05). |
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| Keywords: | chitosan doxorubicin drug loading encapsulation human ovarian cancer iron oxide |
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