Effect of double growth factor release on cartilage tissue engineering |
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Authors: | Ayşe Burcu Ertan Pınar Yılgor Banu Bayyurt Ayşe Ceren Çalıkoğlu Çiğdem Kaspar Fatma Neşe Kök Gamze Torun Kose Vasif Hasirci |
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Affiliation: | 1. Department of Genetics and Bioengineering, Yeditepe University, Faculty of Engineering and Architecture, , Istanbul, Turkey;2. Department of Biochemistry, Cukurova University Faculty of Medicine, , Adana, Turkey;3. BIOMATEN Centre of Excellence in Biomaterials of Tissue Engineering, Biotechnology Research Unit, Middle East Technical University, , Ankara, Turkey;4. Department of Molecular Biology and Genetics, Biotherapeutic ODN Lab, Bilkent University, , Ankara, Turkey;5. Department of Medicine, Yeditepe University, , Istanbul, Turkey;6. Molecular Biology and Genetics Department, Istanbul Technical University, , Istanbul, Turkey;7. Department of Biological Sciences, Middle East Technical University, , Ankara, Turkey |
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Abstract: | The effects of double release of insulin‐like growth factor I (IGF‐I) and growth factor β1 (TGF–β1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid‐co‐glycolic acid) (PLGA) and poly(N‐isopropylacrylamide) (PNIPAM) carrying IGF‐I and TGF–β1, respectively. On tissue culture polystyrene (TCPS), TGF‐β1 released from PNIPAM nanoparticles was found to have a significant effect on proliferation, while IGF‐I encouraged differentiation, as shown by collagen type II deposition. The study was then conducted on macroporous (pore size 200–400 µm) PLGA scaffolds. It was observed that the combination of IGF‐I and TGF‐β1 yielded better results in terms of collagen type II and aggrecan expression than GF‐free and single GF‐containing applications. It thus appears that gradual release of a combination of growth factors from nanoparticles could make a significant contribution to the quality of the engineered cartilage tissue. Copyright © 2011 John Wiley & Sons, Ltd. |
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Keywords: | cartilage tissue engineering growth factors peptide and protein delivery mesenchymal stem cells cell differentiation |
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