In vitro IL-6 production by EBV-immortalized B lymphocytes from young and elderly people genotyped for -174 C/G polymorphism in IL-6 gene: a model to study the genetic basis of inflamm-aging |
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Authors: | Olivieri Fabiola Bonafè Massimiliano Giovagnetti Simona Stecconi Rosalia Cardelli Maurizio Cavallone Luca Spazzafumo Liana Marchegiani Francesca Carrieri Giuseppina Mugianesi Elena Giampieri Claudia Centurelli Matteo Moresi Raffaella Tesei Silvia Lisa Rosamaria Viticchi Claudio Falsetti Lorenzo Salvioli Stefano Franceschi Claudio |
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Institution: | Italian National Research Centers on Aging (I.N.R.C.A.), Via S. Margherita, n. 5, 60121, Ancona, Italy. |
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Abstract: | In the present investigation we analysed Interleukin 6 (IL-6) in vitro production by Epstein-Barr virus (EBV)-immortalized B lymphocytes established from 43 subjects, 15 young people and 28 elderly people, including 18 centenarians, after 3, 6, 9, 24, 48 and 72 h of culture. The subjects were genotypized for the C to G transition at nucleotide -174 of IL-6 gene promoter (-174 C/G) and were classified as C allele carriers (C+) and non-carriers (C-). We found that: (i) the interindividual difference in in vitro IL-6 production was wider in elderly individuals in respect to young individuals, leading to different coefficient of variation in the two groups; (ii) the -174 C/G polymorphism had an age-related effect on IL-6 in vitro production. Only among C- people, cells from elderly subjects produced significant higher level of IL-6 than cells from young subjects. These data are consistent with our previous results regarding the IL-6 serum levels in a large group of people of different age, including centenarians. Thus, the EBV-immortalized B lymphocytes can be considered a useful in vitro model for studying the genetic control of IL-6 production and its changes with age. |
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Keywords: | IL-6 polymorphism EBV-immortalized B lymphocytes Centenarians |
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