不同浓度一氧化氮对冻干移植骨修复骨缺损影响的实验研究

目的　比较深低温冷冻干燥骨和亚硝基化牛血清蛋白的复合物与单独应用深低温冷冻干燥骨在修复大段骨缺损中的效果 ,探讨NO在异体组织移植中的作用。方法　采用新西兰大耳白兔桡骨中段切除 1.5cm骨干和骨膜的实验动物模型 ,同时制备能在体内持续缓慢释放一氧化氮的亚硝基化牛血清蛋白 ,将 4 8只兔随机分成 3组。A组 :低浓度亚硝基化牛血清蛋白与深低温冷冻干燥骨的复合物 (低浓度NO冻干骨组 ) ;B组 :高浓度亚硝基化牛血清蛋白与深低温冷冻干燥骨的复合物 (高浓度NO冻干骨组 ) ;C组 :深低温冷冻干燥骨 (单纯冻干骨组 )。分别在术后3、6、9及 12周对上述 3组的骨缺损模型进行X线片、组织学检查及钙含量测定。并在 12周处死动物前 ,测量动物体内NO的含量。结果 (1)低浓度NO冻干骨组修复骨缺损的效果明显好于单纯冻干骨组 (P <0 .0 5 )和高浓度NO冻干骨组 (P <0 .0 1) ,术后第 12周缺损区完全性修复 ,骨髓腔再通 ;单纯冻干骨组修复骨缺损的效果优于高浓度NO冻干骨组 (P <0 .0 5 )。 (2 ) 12周时高浓度NO冻干骨组的NO含量高于单纯冻干骨组 (P <0 .0 1)和低浓度NO冻干骨组 (P <0 .0 5 ) ,低浓度NO冻干骨组的NO含量高于单纯冻干骨组 (P <0 .0 5 )。结论　在修复骨缺损的过程中 ,低浓度NO能促进骨愈合 ,而高浓度N

An Experimental Study on Repairing Bone Defects with Different Concentration Nitric Oxide Combined with Freeze-dried Bone Allograft

Objective To evaluate the effect of different concentration Nitric Oxide(NO) on repairing of large segemental bone defects. Methods Segemental bone defects for 15mm were created in the 48 New Zealand rabbits on mid part of the right radius, and Nitroso-bovine serum albumin was made as nitric oxide donor.48 rabbits were divided into three groups,the defects being treated with three kinds of implants, A: low concentration nitroso-bovine serum albumin combined with.freeze-dried bone allograft, B: high concentration nitroso-bovine serum albumin combined with freeze-dried bone allograft, C: single free-dried bone allograft as control. Radiographic and histologic examination was made and the amount of calcium in the defect area was tested at 3,6,9 and 12 weeks after operation. 12 weeks before killing the rabbits, the content of nitrite in the rabbits body was measured. Results The results showed Group A was better than Group B(P <0.01) and Group C(P <0.05) in the process of bone healing,and Group C was better than Group B(P <0.05).12 weeks after surgery,mean serum nitrite levels in Group B were higher than in Group A(P <0.05) and in Group C(P <0.01),the levels in Group A were higher than in Group C(P <0.05).By 12 weeks, the defects in Group A were repaired completely and the medullary canal was recanalized. Conclusion Low concentration applied NO with NO donor nitroso-bovine serum provided beneficial effects to bone healing, while high concentration Nitric Oxide had restraining effects in the process of bone healing.