卡托普利对病毒性心肌炎小鼠心肌线粒体结构及其酶活性的影响

目的　研究卡托普利对病毒性心肌炎小鼠心肌线粒体结构和三磷酸腺苷 (ATP)酶活性变化的影响。方法　将雄性Balb/c小鼠随机分为柯萨奇病毒B3 (CVB3 )感染组、CVB3 感染加卡托普利治疗组和对照组。以d3、d14为两个时相点 ,比较线粒体超微结构 ,线粒体Na+ K+ ATP酶、Ca2 + ATP酶活性变化。结果　感染组心肌细胞线粒体结构破坏 ,线粒体Na+ K+ ATP酶、Ca2 + ATP酶活性较对照组显著下降 (P均 <0 .0 1)。卡托普利治疗组各时相点线粒体结构破坏较轻 ,线粒体Na+ K+ATP 酶、Ca2 + ATP酶活性较感染组明显增高 (P均 <0 .0 5 )。结论　病毒性心肌炎早期即可见心肌线粒体结构破坏 ,ATP酶活性下降。卡托普利可有效保护心肌线粒体结构和功能

The effect of carptopril on myocardial mitochondrial structure and activity of adenosine triphosphatase in mice with myocarditis

Objective To explore changes of myocardial mitochondrial structure and activity of adenosine triphosphatase(ATPase) in mice with myocarditis,and observe the intervention effect of carptopril.Methods The male Balb/c mice were randomly divided into 3 groups: the coxsackie B 3 virus(CVB 3)infected group,the infected CVB 3 and treated with carptopril group,control group. The activity of Na +-K +- ATPase and Ca 2+ -ATPase of myocardial mitochondrial as well as the morphological of myocardial mitochondrial were detected on 3 day and 14 day.Results In the infected group the activity of mitochondrial Na +-K +- ATPase and Ca 2+ -ATPase significantly declined ( P <0.01), the structure of mitochondrial changed distinctly in different periods. All these were significantly improved in the infected CVB 3 and treated with carptopril group( P <0.05).Conclusions The damage of structure and decline of ionic pump functions in myocardial mitochondrial can be observed in early stage of myocarditis. Carptopril exerts a favorable effect on myocardial mitochondria.