哮喘患者检测痰中基质金属蛋白酶的临床意义

目的探讨支气管哮喘（简称哮喘）患者诱导痰中基质金属蛋白酶9（MMP-9）和基质金属蛋白酶抑制剂1（TIMP-1）的浓度比值与气道炎症和气流受限的关系。方法选择缓解期哮喘患者（哮喘组）和健康对照者（健康对照组）进行肺功能测定；用诱导痰检查方法对痰炎性细胞进行分类计数，并用酶联免疫吸附试验（ELISA）法测定诱导痰上清液中MMP-9和TIMP-1浓度。结果哮喘组和健康对照组诱导痰中MMP-9、TIMP-1浓度比较差异和MMP-9／TIMP-1比值比较差异有统计学意义。结论哮喘组患者诱导痰中MMP-9／TIMP-1比值的失衡与气道炎症有关，从而造成气道重塑和气流受限，导致肺功能下降。

Clinical significance of test of metalloproteinase in sputum from asthma patients

Objective To investigate the relationship between the levels of metalloproteinase-9 （MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in induced sputum in patients with asthma and the airway inflammation and airflow limitation. Methods Sixteen patients with asthma in remission sta- ges, and twelve normal control subjects were included in this study. Lung function was measured. Induced sputum was obtained and processed for cell differential count. The concentrations of MMP-9 and TIMP-1 in supernatant were assayed by enzyme-linked immunosorbent assay （ELISA）. Results The difference of the concentration of MMP-9/TIMP-1 in induced sputum and the difference of the ratio of MMP-9/TIMP-1 in asthmatics and in normal control subjects were statistical analyzed. Conclusion The imbalance between MMP-9 and TIMP-1 in induced sputum in asthmatics is associated with airway inflammation and airflow llml- tation, which may play a role in the pathogenesis of extracellular matrix remodeling and airflow limitation.