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| 双向电泳-免疫印迹技术分离白血病细胞核基质蛋白的初步研究 | |
| 引用本文： | 刘峰,陈元玉,李冠武.双向电泳-免疫印迹技术分离白血病细胞核基质蛋白的初步研究[J].汕头大学医学院学报,2004,17(3):151-153. |
| 作者姓名： | 刘峰 陈元玉 李冠武 |
| 作者单位： | 1. 汕头大学医学院第二附属医院血液科,广东,汕头,515041 2. 汕头大学医学院第二附属医院外科 3. 汕头大学医学院生化教研室 |
| 基金项目： | 广东省卫生厅资助课题 (A2 0 0 0 4 68) |
| 摘 要： | 目的：探讨急性髓系细胞白血病核基质蛋白(NMPs)的变化。方法：高盐抽提法制备白血病细胞NMPs。免疫动物制备多克隆抗体，以此作为免疫学探针对正常WBC和白血病细胞NMPs进行双向电泳(2DE)-免疫印迹(Western blot)检测。结果：在急性髓系细胞白血病细胞和正常WBC NMP8之间，有3个蛋白质表现出质和量上的差异。结论：2DE-Western blot方法能较好地分辨出正常WBC和白血病细胞NMPs的差异。 |
| 关 键 词： | 白血病细胞 正常 核基质蛋白 WBC 髓系 急性 免疫印迹技术 双向电泳 初步研究 多克隆抗体 |
| 文章编号： | 1007-4716(2004)03-0151-03 |
| 修稿时间： | 2004年6月22日 |
Preliminary Study on Nuclear Matrix Proteins in Leukemia Cells by Two-dimensional Electrophoresis and Western Blot |
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| LIU Feng.Preliminary Study on Nuclear Matrix Proteins in Leukemia Cells by Two-dimensional Electrophoresis and Western Blot[J].Journal of Shantou University Medical College,2004,17(3):151-153. | |
| Authors: | LIU Feng |
| Abstract: | Objective:To study the changes of nuclear matrix proteins(NMPs)in leukemia cells.Methods:NMPs were prepared by high salt extraction and immunized rabbit to raise polyclonal antibody which was used to detect the membrance transferred from 2 D gel of nuclear matrix in normal WBC and acute myeloid leukemia cells by Western blot.Results:There were 3 proteins spots appeared with difference between normal WBC and leukemia cells.Conclusion:2DE-Western blot is an available method to explore the difference between normal WBC and leukemia cells. |
| Keywords: | Nuclear Matrix Leukemia 2-dimensional Electrophoresis |
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