肺结核患者外周血T淋巴细胞亚群与核仁组成区嗜银蛋白的关系及临床应用的研究

目的　探讨肺结核患者T淋巴细胞亚群与核仁组成区嗜银蛋白(AgNORs)的关系,并动态观察临床应用的价值。方法 采用碱性磷酸酶抗碱性磷酸酶(APAAP)法及AgNORs测定方法,对98例肺结核患者,39例肺炎患者和41名正常人分别测定外周血T淋巴细胞亚群和AgNORs,并对71例初治肺结核患者进行了动态观察。结果 肺结核患者的CD₃⁺、CD₄⁺、CD₄⁺/CD₈⁺比值均明显降低,CD₈⁺升高；AgNORs的I.S%值明显降低,与健康人及肺炎患者相比有显著性差异(P<0.01)。随着肺结核患者病情的好转此两项指标逐渐上升。结论 肺结核患者T淋巴细胞亚群与AgNORs检测结果一致,可动态地反映结核病人的免疫状态,并与病情发展呈良好的相关性,可作为指导治疗的参考。

Study on clinical application and correlation of T lymphocyte subgroup and argyrophilic proteins of the nucleolar organizer regions in patients with pulmonary tuberculosis

Objective To explore clinical significance and relationship of T lymphocyte subgroup and argyrophilic protein in the nucleolar organizer regions (AgNORs) in pulmonary tuberculosis patients.Methods 98 patients with pulmonary tuberculosis,39 patients with pneumonia and 41 healthy people were included by analyzing their T lymphocyte subgroup with the alkaline phosphatase-antialkaline phosphatase(APAAP) method and the areas of AgNORs in T cell with a KL Imaging System. 71 patients with pulmonary tuberculosis were observed continually during treatment.Results CD₃⁺,CD₄⁺,CD₄⁺/CD₈⁺ ,I.S% of patients with pulmonary,tuberculosis were significantly lower than those of patients with pneumonia and healthy people (P<0.01). When tuberculosis patients’condition was getting better, these two values also were readings going up.Conclusion The Results of T lymphocyte subgroup are in accord with those of AgNORs. These Results show immune function of patients and can be used as reference of guiding treatment.