| Use of autologous tissue engineered skin to treat porcine full-thickness skin defects |
| |
| 引用本文： | 蔡霞 曹谊林 崔磊 刘伟 关文祥. Use of autologous tissue engineered skin to treat porcine full-thickness skin defects[J]. 中华创伤杂志(英文版), 2005, 8(5): 269-276 |
| |
| 作者姓名： | 蔡霞 曹谊林 崔磊 刘伟 关文祥 |
| |
| 作者单位： | Department of Plastic Reconstructive Surgery，Shanghai Ninth People's Hospital，The Key Laboratory of Tissue Engineering，Shanghai Second Medical University，Shanghai200011，China，Department of Plastic Reconstructive Surgery，Shanghai Ninth People's Hospital，The Key Laboratory of Tissue Engineering，Shanghai Second Medical University，Shanghai200011，China，Department of Plastic Reconstructive Surgery，Shanghai Ninth People's Hospital，The Key Laboratory of Tissue Engineering，Shanghai Second Medical University，Shanghai200011，China，Department of Plastic Reconstructive Surgery，Shanghai Ninth People's Hospital，The Key Laboratory of Tissue Engineering，Shanghai Second Medical University，Shanghai200011，China，Department of Plastic Reconstructive Surgery，Shanghai Ninth People's Hospital，The Key Laboratory of Tissue Engineering，Shanghai Second Medical University，Shanghai200011，China |
| |
| 摘 要： | Iinn irteiaceln ta uyteoagrrsa,ft sk iann dgra fatlilnoggr hafats e pvroelvpaedra tfiroonms th toebiosynthetic and tissue-engineered living skinreplacements.The production of bioengineered humantissues has led to fascinating diversity of medicalapplications,notably for permanent burn woundcoverage.1For a few decades,various skin substituteshave been introduced for extensive full-thickenss burnmanagement,and also provided new approaches forreconstructive surgery.2-7In addition,allografting ofcu…
|
| 关 键 词： | 自体同源组织 皮肤组织工程 治疗方法 猪 动物实验 皮肤缺损 |
Use of autologous tissue engineered skin to treat porcine full-thickness skin defects |
| |
| Cai Xia,CAO Yi-lin,CUI Lei,LIU Wei,GUAN Wen-xiang. Use of autologous tissue engineered skin to treat porcine full-thickness skin defects[J]. Chinese journal of traumatology, 2005, 8(5): 269-276 |
| |
| Authors: | Cai Xia CAO Yi-lin CUI Lei LIU Wei GUAN Wen-xiang |
| |
| Affiliation: | Department of Plastic Reconstructive Surgery, Shanghai Ninth People's Hospital, The Key Laboratory of Tissue Engineering, Shanghai Second Medical University, Shanghai 200011, China |
| |
| Abstract: | Objective: To explore a feasible method to repair full-thickness skin defects utilizing tissue engineered techniques. Methods: The Changfeng hybrid swines were used and the skin specimens were cut from the posterior limb girdle region, from which the keratinocytes and fibroblasts were isolated and harvested by trypsin, EDTA, and type II collagenase. The cells were seeded in Petri dishes for primary culture. When the cells were in logarithmic growth phase, they were treated with trypsin to separate them from the floor of the tissue culture dishes. A biodegradable material, Pluronic F-127, was prefabricated and mixed with these cells, and then the cell-Pluronic compounds were seeded evenly into a polyglycolic acid (PGA). Then the constructs were replanted to the autologous animals to repair the full-thickness skin defects. Histology and immunohistochemistry of the neotissue were observed in 1, 2, 4, and 8 postoperative weeks. Results: The cell-Pluronic F-127-PGA compounds repaired autologous full-thickness skin defects 1 week after implantation. Histologically, the tissue engineered skin was similar to the normal skin with stratified epidermis overlying a moderately thick collageneous dermis. Three of the structural proteins in the epidermal basement membrane zone, type IV collagen, laminin, and type VII collagen were detected using immunohistochemical methods. Conclusions: By studying the histology and immunohistochemistry of the neotissue, the bioengineered skin graft holds great promise for improving healing of the skin defects. |
| |
| Keywords: | Tissue engineering Skin Keratinocytes Fibroblasts |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
