特应性皮炎患者外周血辅助性T细胞22的表达及意义

目的]检测特应性皮炎(AD)患者外周血辅助性T细胞22(Th22)的表达,探讨其在AD发病机制中的作用.方法]以湿疹面积及严重度指数(EASI)评分评估患者病情,采用流式细胞术检测46例AD患者和28例健康对照者外周血Th22(IL-22)细胞,ELISA法检测血清中IL-22、IFN-γ、IL-4水平.结果]AD患者外周血Th22细胞(2.35±0.64)％,明显高于对照组的(0.94±0.43)％(P＜ 0.01); Th22细胞与血清中IL-22呈正相关(r=0.48,P＜0.05),而与IFN-γ、IL-4无相关(r=- 0.105,P＞0.05；r=- 0.267,P＞0.05),Th22细胞与疾病的EASI评分呈正相关(r=0.53,P＜0.05).结论]Th22细胞有可能作为监测AD严重程度或病情波动的指标.

Expression and significance of peripheral blood T helper cell 22 in patients with atopic dermatitis

Objective To detect the expression of peripheral blood T helper cell 22 （Th22） in patients with atopic dermatitis （AD）, and approach its role in AD pathogenesis. Methods The patients＇ conditions were evaluated by Eczema area and severity index （EASI） score. The peripheral blood Th22 （IL-22） cells of 46 patients with AD and 28 healthy controls were detected by flow cytometry. Serum IL-22, IFN- 3, and IL-4 level were detected by ELISA assay. Results Th22 cells in peripheral blood of AD patients （2.35 ± 0.64）% were significantly higher than those in the control group （0.94 ± 0.43）% （P〈 0.01）. There was a positive correlation between Th22 cells and serum IL-22 （r=0.48, P 〈 0.05） and no correlation between Th22 ceils and IFN- γ , IL-4 （r=0.105, r=-0.267, P〉 0.05）, Th22 cells and EASI score of the disease was positively correlated （r=0.53, P 〈 0.05）. Conclusion Th22 cells may be used as the index of monitoring AD severity or disease fluctuations.