白藜芦醇抑制胆囊癌细胞生长与诱导细胞凋亡的实验研究

目的:探讨白藜芦醇(Res)对胆囊癌细胞(GBC)和正常成纤维细胞(3T3)体外增殖的影响,进而观察Res对GBC和3T3细胞凋亡的影响.方法:MTT法测定肿瘤细胞生长抑制率;流式细胞术分析细胞周期,检测细胞凋亡;SABC法检测细胞bcl-2、c-myc、p53蛋白表达.结果:Res呈浓度依赖性抑制GBC细胞的生长与增殖(P＜0.01),抑制率最高可达54%.Res能明显诱导GBC细胞凋亡,凋亡率最高为30.52%;处理组较对照组G1期细胞由34.88%上升至55.47±3.95%,S期细胞减少8.41%～17.54%,呈明显的G0/G1期阻滞现象.GBC细胞的bcl-2、c-myc基因蛋白表达降低,而p53基因蛋白表达增强.结论:Res能通过诱导GBC细胞凋亡而抑制其生长与增殖,但对3T3细胞无此作用.

Research on Resveratrol's Effects on Suppressing Growth and Inducing Apoptosis of GBC Cells

OBJECTIVE: To explore the contribution of resveratrol (Res) to the proliferation and apoptosis of gallbladder carcinoma (GBC) cell line as well as fibroblast 3T3 cell line in vitro. METHODS: The tumor cell growth repressing rate was measured by the MTT method,and flow cytometry was used to analyse cell cycle. The gene expression of bcl-2 c-myc p53 were examined by SABC. RESULTS: Res obviously suppressed the proliferation (P <0. 01) in concentration and time dependent manner, it's tiptop was 54%. Res could induce apoptosis of tumor cells. The maximum apoptosis rate of tumor was 30. 52%, experiment group compared with comparison group Gl's cells rised from 34. 88% to 55.47 +/- 3. 95%, S's cells reduced 8.41 - 17.54%, which showed obvious phenomenon of G0/G1 blocking. The GBC cell's bcl-2, c-myc gene reduced expression,while p53 gene increased expression. CONCLUSION: The results of this study confirm the ability of Res to suppress the proliferation of gallbladder carcinoma (GBC) cell with a typical apoptotic feature in vitro. Therefore, Res may be considered as a possible treatment strategy for gallbladder carcinoma.