| 三叶青提取物抗乙肝病毒活性的研究 |
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| 作者姓名： | 杨雄志 巫军 |
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| 作者单位： | 1. 浙江医药高等专科学校中药系,浙江宁波,315200
2. 宁波拜奥生物研究所,浙江宁波,315201 |
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| 基金项目： | 浙江省宁波市科研攻关基金 |
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| 摘 要： | 目的 研究三叶青4个提取部位对乙肝病毒的抑制作用.方法 运用mT法检测样品药物导致50%细胞死亡的数值浓度为CC50;用ELISA方法检测样品药物达到抑制HbsAg、HbeAg分泌,以及运用荧光定量PCR法检测样品药物抑制病毒DNA复制量的50%时的浓度数值为IC50.结果 三叶青4个提取部位对乙肝病毒分泌的HbsAg、HbeAg均有抑制作用.结论 三叶青乙酸乙酯部位对乙肝病毒具有较强活性,能明显降低HBV的DNA复制水平,可进一步从中提取分离具有更强生物活性的物质,为新药开发提供物质基础.
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| 关 键 词： | 三叶青 HepG2.2.15细胞 抗乙肝病毒 |
A Study of Anti-HBV Activity of Extract of Radix Tetrastigmae |
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| Authors: | YANG Xiong-zhi WU Jun |
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| Institution: | 1Department of Chinese Medicine;Zhejiang Medical College;Ningbo;Zhejiang;315200;China;2Ningbo Biomedical Research Institute;315201;China |
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| Abstract: | To study the effect of four fractions extracted from Radix Tetrastigmae on HBV.METHOD: The level of HBsAg and HBeAg secreted from HepG2.2.15 cells and the copies of DNA which were treated with four samples were detected.RESULT The four fractions from Radix Tetrastigmae showed inhibition activity on HBV.CONCLUSION The ethyl acetate part of Radix Tetrastigmae can significantly restrain the secretion of HBsAg and HbeAg from HepG2.2.15 cells,which can be further researched as new anti-HBV drugs. |
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| Keywords: | Radix Tetrastigmae HepG2 2 15 cells anti-HBV |
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