| 乳腺癌细胞MDA-MB-231肺转移模型的建立及其评价 |
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| 引用本文： | 曹让娟,翟艳华,卿旭,刘雅文,朱筱娟.乳腺癌细胞MDA-MB-231肺转移模型的建立及其评价[J].吉林大学学报(医学版),2011,37(3):403-406. |
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| 作者姓名： | 曹让娟 翟艳华 卿旭 刘雅文 朱筱娟 |
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| 作者单位： | 东北师范大学遗传与细胞研究所神经可塑性实验室,吉林,长春,130024;吉林大学公共卫生学院流行病与卫生统计学教研室,吉林,长春,130021 |
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| 基金项目： | 国家自然科学基金资助课题(30871302,30870952); 吉林省科技厅自然科学基金资助课题(200805130) |
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| 摘 要： | 目的:探讨用绿色荧光蛋白(GFP)标记的乳腺癌细胞MDA-MB-231建立可量化评估的自发性肺转移和实验性肺转移动物模型,为研究乳腺癌的转移机制提供依据.方法:采用慢病毒载体介导的病毒包装体系,获得稳定表达GFP的细胞系MDA-MB-231-GFP,通过皮下注射方式将细胞接种于Balb/C裸鼠皮下,建立自发性肺转移模型...
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| 关 键 词： | 乳腺肿瘤 慢病毒体系 肺转移 动物模型 |
| 收稿时间： | 2011-01-27 |
Establishment and evaluation of mouse model for lung metastasis of breast cancer cell MDA-MB-231 |
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| CAO Rang-juan,ZHAI Yan-hua,QING Xu,LIU Ya-wen,ZHU Xiao-juan.Establishment and evaluation of mouse model for lung metastasis of breast cancer cell MDA-MB-231[J].Journal of Jilin University: Med Ed,2011,37(3):403-406. |
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| Authors: | CAO Rang-juan ZHAI Yan-hua QING Xu LIU Ya-wen ZHU Xiao-juan |
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| Institution: | (1. Laboratory of Neural Plasticity,Institute of Genetics and Cytology,Northeast Normal University,Changchun 130024,China; |2. Department of Epidemiology and Health Statistics,School of Public Health,Jilin University,Changchun 130021,China) |
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| Abstract: | Objective To establish quantifiable primary and experimental lung metastasis mouse models by using GFP labeled MDA-MB-231 cell lines and provide basis for the study on metastasis mechanism of breast cancer.Methods The MDA-MB-231 cells were infected by GFP labeling lentiviral vector-mediated packaging system and inoculated subcutaneously into Balb/C nude mice or through tail vain into SCID mice to set up primary and experimental lung metastasis models.Then the mice were executed at 8 th and 5 th weeks,and th... |
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| Keywords: | breast neoplasm lentivirus system lung metastasis animal model |
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