磷霉素与氨基糖苷类药物联用对铜绿假单胞菌生物被膜的影响

目的通过研究磷霉素与氨基糖苷类药物(阿米片星、异帕米星)联合应用对铜绿假单胞菌生物被膜的体外联合效应,探讨磷霉素对氨基糖苷类药物在抑制铜绿假单胞菌生物被膜形成的作用。方法采用改良的组织培养平板法建立铜绿假单胞菌(PAO1)生物被膜模型,应用扫描电镜观察经不同剂量的两类药物单用与联合作用处理后的PAO1生物被膜结构,对其形态学进行分析比较。结果扫描电镜的形态学结果显示,PAO1细菌呈杆状,为浓厚的粘液样物质紧密包绕,聚集成团,菌体之间相互粘连,生物被膜形态完整。经不同浓度的磷霉素、阿米卡星、异帕米星、磷霉素+阿米卡星、磷霉素+异帕米星处理后,随着药物剂量的增加,细菌数减少,聚集程度降低,细菌间粘液状物显著减少。磷霉素+阿米卡星和磷霉素+异帕米星联用的抑制被膜形成作用基本相似,磷霉素的抑制被膜形成作用较阿米卡星和异帕米星差,联药组的抑制被膜形成作用较单药组显著。结论磷霉素与氨基糖苷类药物联用对铜绿假单胞菌生物被膜形成的抑制具有显著增效作用。

The Effect of the Combination of Fosfomycin and Aminoglycosides on Pseudomonas aeruginosa Biofilm

Objective To study the combination effect of fosfomycin and aminoglycosides on the formation of biofilm ofPseudomonas aerug/nosa（PAO1） in vitro. Methods The modified plate culture method was used to establish biofilm model in vitro with which the morphology of PAO1 biofilms were observed and compared by scanning electron microscopy（SEM） after treated with different dosages of fosfomycin and aminoglycosides. Results SEM of PAO1 biofilms showed that bacteria were both bacillus surrounded by accidented material and the bacteria were connected each other with mucoid substance. The restraining effects on the formation of bioKlm of combination groups were more than that of non-combination groups. Conclusion There were obvious differences between combination of fosfomycin and aminoglycosides groups and non-combination groups in the formation of PAO1 biofilm. Synergistic action of fbsfomycin to aminoglycosides is verified.