3种雷公藤制剂对大鼠急性毒性损伤的比较

目的观察并比较临床常用3种雷公藤制剂对大鼠的急性毒性。方法将Wistar大鼠随机分为对照组、雷公藤片组、雷公藤多苷片组、昆明山海棠片组，各组分别灌胃给予相应的人日用量60倍药物，观察一般症状，检测血清谷草转氨酶（AST）、谷丙转氨酶（ALT）、血清尿素氮（BUN）值，肉眼观察心、肝、脾、肺及肾的组织形态并计算脏器指数。对肝肾进行HE染色，观察并比较各组的病理损伤程度。结果与对照组相比，雷公藤片组、雷公藤多苷片组AST值显著升高（P〈0．05），雷公藤多苷片组ALT值显著升高（P〈0．05），各给药组AST／ALT、BUN变化均不显著（P〉0．05）；各给药组肝、肾组织均出现一定程度病理损伤，其中肾组织损伤更严重，给药组间比较，昆明山海棠片肝肾损伤轻于另外两组。结论3种雷公藤制剂一次性大剂量给予大鼠均出现不同程度的急性中毒症状，造成肝肾损害，雷公藤片及雷公藤多苷片的急性毒性损伤较昆明山海棠片严重，而前两者之间的急性毒性无显著性差异。

Comparative Study on Rat Acute Toxicity of Three Tripterygium Wilfordii Preparations

Objective To observe and compare the acute toxicity of three Tripterygium Wilfordii Preparations. Methods The rats were randomly divided into 4 groups, the control group, Tripterygium wilfordii tablet group, Tripterygium glycosides tablet group, Tripterygium hypoglaucum Hutch group. Rats were given corresponding drugs. General symptom and organs were observed, and AST, ALT, BUN in serum were measured. Liver and renal histology was examined by HE staining. Results The AST of Tripterygium wilFordii tablet group and Tripterygium glycosides tablet group, the ALT of Tripterygium glycosides tablet group were higher than those of the control group（P 〈0.05）. There was no significant difference （P 〉0.05） between medication groups in AST/ALT and BUN. The degree of Tripterygium hypoglaucum Hutch group changes was less than that of two other medication groups. Conclusion The three Tripterygium WilFordii Preparations can induce the renal and liver acute lesion, and the toxicity of the Tripterygium wilfordii tablet and Tripterygium glycosides tablet is more serious than that of Tripterygium hypoglaucum Hutch.