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| 艾叶不同组分发挥镇痛作用的安全范围研究 | |
| 引用本文： | 迟雪洁,王会,黄伟,鲍志烨,孙蓉.艾叶不同组分发挥镇痛作用的安全范围研究[J].中国药物警戒,2012,9(6):330-332. |
| 作者姓名： | 迟雪洁 王会 黄伟 鲍志烨 孙蓉 |
| 作者单位： | 1. 天津中医药大学,天津300193;山东省中医药研究院,山东济南250014 2. 山东中医药大学,山东济南,250355 3. 山东省中医药研究院,山东济南,250014 |
| 基金项目： | 国家973计划中医基础理论专项资助项目（2009CB522802）,山东省科技平台建设项目课题（2008GG2NS02021） |
| 摘 要： | 目的明确艾叶发挥镇痛作用的物质基础和剂量范围,确定相应的安全范围,为临床用药提供依据。方法采用热板法进行艾叶水提组分和挥发油小鼠镇痛作用考察,采用经典的急性毒性方法进行小鼠毒性数据考察,并通过Bliss法计算相关指标。结果艾叶水提组分和挥发油发挥镇痛作用的的半数有效量(ED50)分别为2.0978 g.kg-1.d-1、0.302 mL.kg-1.d-(1折合生药量为39.7 g.kg-1.d-1)ED5095%可信区间为(1.5555~2.7917)g.kg-1.d-1、(0.213~0.429)mL.kg-1.d-1折合生药量为(28.0~56.4)g.kg-1.d-1。艾叶水提组分小鼠镇痛作用的治疗指数(TI)为38.2、安全系数(SF)为10.9,其ED95和LD5分别相当于药典规定的人日用量的46.40和505.60倍。艾叶挥发油的TI为5.53、SF为1.06,其ED95和LD5分别相当于药典规定的人日用量的1086.70和1151.60倍。结论艾叶水提组分和挥发油均是其镇痛物质基础,发挥镇痛作用的剂量范围分别为(1.5555~2.7917)g.kg-1.d-1、(28.0~56.5)g.kg-1.d-1,明确了水提组分较挥发油对小鼠热刺激的反应更灵敏,治疗效果更佳,挥发油较水提组分安全范围较窄、安全性低。 |
| 关 键 词： | 艾叶 镇痛 安全范围 治疗指数 半数有效量 |
The Analgesic Effect Study on Margin of Safety on Different Components from Folium Artemisiae Argyi |
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| CHI Xue-jie , WANG Hui , HUANG Wei , BAO Zhi-ye , SUN Rong.The Analgesic Effect Study on Margin of Safety on Different Components from Folium Artemisiae Argyi[J].Chinese JOurnal of Pharmacovigilance,2012,9(6):330-332. | |
| Authors: | CHI Xue-jie WANG Hui HUANG Wei BAO Zhi-ye SUN Rong |
| Institution: | CHI Xue-jie WANG Hui HUANG Wei BAO Zhi-ye SUN Hong. (1Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; 2Shandong Research Academy of TCM, Shandong Jinan 250014, China; 3Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China) |
| Abstract: | Objective To determine the material basis of analgesic effect and the effective dose range of Fohum Artemisia Argyi, to clear the appropriate therapeutic index and safety margin, in order to provide the basis for the clinical safety. Methods Analgesic effect of the volatile oil and water extracts from Foh＇um Artemisia Argyi were investigated by hot plate test and the toxicity in mice were investigated by the Bliss method to calculate the relevant indicators. Results The ED50 and 95% confidence limit of water extraction components and volatile oil in Artemisia Argyi are respectively 2.0978 g·kg-1·d-1（1.5555 - 2.7917） g·kg-1·d-1, 0.302 mL·kg-1·d-1（0.213 - 0.429）mL·kg-1·d-1. TI and SF of water extraction components are respectively 38.2,10.9. TI and SF of volatile oil volatile oil in Artemisia Argyi are respectively 5.53, 1.06. ED65 and LD5 are respectively 1 086.70 and 1 151.60 amounts equivalent to the pharmacopoeia. Conclusion The material basis of Folium Arterrdsia Argyi are water extraction components and volatile oil, the effective dose range are respectively（1.5555 - 2.7917）g·kg-1·d-1, （28.0 - 56.5）g ·kg-1·d-1. This study cleared water extraction components were more sensitive than volatile oil on the response to thermal stimulation in mice. The volatile oil has narrower margin of safety and low security than water extraction components. |
| Keywords: | Folium Arterrdsia Argyi analgesic margin of safety therapeutic index ED50 |
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