鸦胆子不同组分发挥抗炎作用的安全范围研究

目的 研究鸦胆子不同组分发挥抗炎作用的治疗指数和安全范围.方法 通过建立巴豆致小鼠耳肿胀模型和琼脂致小鼠肉芽肿胀模型探究鸦胆子不同组分抗急、慢性炎症作用,并进行鸦胆子不同组分急性致死量实验.计算各组分发挥抗炎药效的ED50和急性毒性试验的LD50和LD5,求得不同组分发挥抗炎作用的治疗指数(TI),安全系数(SF).结果 鸦胆子不同组分对炎症模型有明显的抗炎作用,并呈现一定的量效关系,且水提组分抗炎作用大于醇提组分.鸦胆子水提、醇提组分对耳肿胀模型小鼠的抗炎TI、SF分别为35.6207、7.8040和25.3285、3.6645；鸦胆子水提、醇提组分对肉芽肿模型小鼠的抗炎TI、SF分别为47.6659、7.5494和32.6431、4.2959.结论 鸦胆子醇提组分发挥抗炎作用的安全范围大于水提组分,在应用鸦胆子水提组分时更应密切注意不良反应的发生并加强用药监测.

The Margin of Safety Study on Anti-inflammatory Effect of Different Components from Fructus Bruceae

Objective To research therapeutic index（TI） and the margin of safety factor（SF） of anti-inflammatory effect of Fructus Bruceae. Methods Establish tumidness model induced by daubing croton oil and granuloma model induced by subcutaneous injection of agar to explore acute and chronic anti-inflammatory effect of different components from Fructus Bruceae, and acute lethal dose test is also proceeded. Calculate ED50 and ED9s of anti-inflammatory efficacy and LDs0 and LDs of acute toxicity test of the different components from Fructus Bruceae. And then calculate the therapeutic index（TI） and safety factor（SF） of different components from Fructus Bruceae. Results The different components from Fructus Bruceae showed good anti-inflammatory effect and certain dose-effect relationship. The anti-inflammatory effect of alcohol extraction component was better than it of water extraction component. TI of anti-acute inflammatory effect of the water extraction component and the alcohol extraction component from Fructus Bruceae is 35.6207 and 25.3285, and SF is 7.8040 and 3.6645 respectively. And TI of anti-chronic inflammatory effect of the water extracts and the alcohol extracts from Fructus Bruceae is 47.6659 and 32.6431, and SF is 7.5494 and 4.2959 respectively. Conclusion The anti-inflammatory effect of the water extracts is greater than the alcohol extracts from Fructus Bruceae, but the margin of safety from alcohol extracts is greater than the water extracts from Fructus Bruceae. We should pay more attention to the occurrence of adverse reactions and strengthen drug moni- toringin in the application of the water extracts from Fructus Bruceae.