香加皮不同组分发挥镇痛作用的安全范围研究

目的研究香加皮不同组分发挥镇痛作用的安全范围。方法用热板法观察香加皮不同组分镇痛作用,依据本实验室前文报道的香加皮不同组分急性毒性研究结果,用Bliss法分别计算各组分发挥镇痛药效的ED50、ED95、和急性毒性的LD50,LD5,求得香加皮不同组分发挥镇痛作用的治疗指数(TI),安全系数(SF),以此评价其发挥镇痛作用的安全范围。结果香加皮不同组分均有明显的镇痛作用,呈现明显的量效关系,不同组分发挥镇痛作用的安全范围有所不同,香加皮水提提组分ED50=1.5698 g.kg-1.d-1,TI=59.611,SF=17.4702,其ED95和LD5分别相当于药典规定的人日用量的48.0和839.0倍。香加皮醇提组分ED50=1.3687 g.kg-1.d-1,TI=44.853,SF=14.2405,其ED95和LD5分别相当于药典规定的人日用量的35.7和509.0倍。结论香加皮水提组分发挥镇痛作用的安全范围大于醇提组分,在应用香加皮醇提组分时更应密切注意不良反应的发生并加强用药监测。

The Analgesic Effect Study on Margin of Safety on Different Components from Cortex Periplocae

Objective To research analgesic effect of the margin of safety of Cortex Periplocae. Methods Analgesic effec from different components from Cortex Periplocae were investigated by hot plate test; the symptoms in mice wer observed, the experimental datas of ED50, ED65, LD50 and LD5 are calculated by the Bliss method and the therapeuti index（Tl）, safety factor（SF） of different components from Cortex Periplocae were assured by applying some formula, Results The different components from Cortex Peziplocae showed good effect of analgesia, and showed certai dose-effect relationship, ED50 of the water extracts from Cortex Peziplocae is 1,5698 g·kg-1·d-1, TI is 59.611 and SF is 17.4702, ED65 is equal to 48.0 times and LDs is equal to 839.0 times of 70kg people＇s daily dried medicinal herl expenses. The alcohol extracts from Cortex Periplocae of ED50 is 1.3687 g·kg-1·d-1, TI is 44.853 and SF is 14.2405 ED65 is equal to 35.7 times and LD5 is equal to 509.0 times of 70kg people＇s daily dried medicinal herb expenses Conclusion The analgesic effect of the margin of safety from water extracts is greater than the alcohol extracts fron Cortex Periplocae. We should pay more attention to the occurrence of adverse reactions in the application.