山豆根不同组分发挥抗炎作用的安全范围研究

目的研究山豆根不同组分发挥抗炎作用的治疗指数和安全范围。方法通过建立巴豆致小鼠耳肿胀模型和琼脂致小鼠肉芽肿胀模型探究山豆根不同组分抗急、慢性炎症作用,并进行山豆根不同组分急性致死量实验。计算各组分发挥抗炎药效的ED50和急性毒性试验的LD50和LD5,求得不同组分发挥抗炎作用的治疗指数(TI),安全系数(SF)。结果山豆根不同组分对炎症模型有明显的抗炎作用,并呈现一定的量效关系,且水提组分抗炎作用大于醇提组分。山豆根水提、醇提组分对耳肿胀模型小鼠的抗炎TI、SF分别为34.0185、8.9965和52.8432、16.3049;山豆根水提、醇提组分对肉芽肿模型小鼠的抗炎TI、SF分别为34.4115、8.5146和53.8510、18.1641。结论山豆根水提组分对炎症模型的抗炎效果大于醇提组分,但山豆根醇提组分发挥抗炎作用的安全范围大于水提组分。因此,在应用山豆根水提组分时更应密切注意不良反应的发生并加强用药监测。

The Margin of Safety Study on Anti-inflammatory Effect of Different Components from Radix et Rhizome Sophorae Tonkinensis

Objective To research therapeutic index（TI） and the margin of safety factor（SF） of anti-inflammatory effect of Radix et Rh/zome Sophorae Tonkinensis. Methods Establish tumidness model induced by daubing croton oil and granuloma model induced by subcutaneous injection of agar to explore acute and chronic anti-inflammatory effect of different components from Radix et Rhizome Sophozae Tonkinensis, and acute lethal dose test is also proceeded. Calculate EDs0 and ED95 of anti-inflammatory efficacy and LD50 and LD5 of acute toxicity test of the different components from Radix et Rhizome Sophorae Tonkinensis. And then calculate the therapeutic index（TI） and safety factor（SF） of different components from Radix et Rhizome Sophorae Tonkinensis. Results The different components from Radix et Rhizome Sophorae Tonkinensis showed good anti-inflammatory effect and certain dose-effect relationship. The anti-inflammatory effect of alcohol extraction component was better than it of water extraction component. TI of anti-acute inflammatory effect of the water extraction component and the alcohol extraction component from Radix et Rhizome Sophorae Tonkinensis is 34.0185 and 52.8432, and SF is 8.9965 and 16.3049 respectively. And TI of anti-chronic inflammatory effect of the water extracts and the alcohol extracts from Radix et Rhizome Sophorae Tonkinensis is 34.4115 and 53.8510, and SF is 8.5146 and 18.1641 respectively. Conclusion The anti-inflammatory effect of the water extracts is greater than the alcohol extracts from Radix et Rhizome Sophorae Tonkinensis, but the margin of safety from alcohol extracts is greater than the water extracts from Radix et Rhizome Sophorae Tontcmensis. We should pay more attention to the occurrence of adverse reactions and strengthen drug monitoring in the application of the water extracts from Radix er Rhizome Sophorae Tonkinensis.