流式细胞仪检测恶性淋巴瘤患者外周血T细胞亚群的临床研究

目的 探讨流式细胞仪检测恶性淋巴瘤患者外周血T细胞亚群的效果及其与临床病理、肿瘤类型的关系。方法 选取我院于2014年8月-2016年9月收治的98例恶性淋巴瘤患者为研究组,同期选取98例健康体检者为对照组。应用流式细胞仪检测两组外周血T细胞亚群(CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺)并进行比较。结果 研究组CD3⁺、CD4⁺/CD8⁺水平分别为(55.63±11.25),(1.32±0.62),显著低于对照组的(68.96±12.63),(1.59±0.59),差异具有统计学意义(P＜0.05);研究组CD4⁺、CD8⁺水平(33.67±8.14),(26.02±4.67)与对照组的(34.12±8.33),(25.67±4.53)相比差异无统计学意义(P＞0.05)。霍奇金淋巴瘤、非霍奇金淋巴瘤患者CD3⁺、CD4⁺/CD8⁺水平分别为(54.63±11.36),(1.22±0.65)和(55.52±12.02),(1.34±0.71),显著低于对照组的(68.96±12.63),(1.59±0.59),差异具有统计学意义(P＜0.05);霍奇金淋巴瘤、非霍奇金淋巴瘤患者CD4⁺、CD8⁺水平分别为(33.78±8.23),(25.74±4.88)和(33.62±8.74),(26.32±4.85)与对照组的(34.12±8.33),(25.67±4.53)相比,差异无统计学意义(P＞0.05)。Ⅲ~Ⅳ期恶性淋巴瘤患者CD3⁺、CD4⁺、CD4⁺/CD8⁺水平分别为(52.66±12.47),(28.25±6.32),(1.30±0.62)明显低于对照组的(68.96±12.63),(34.12±8.33),(1.59±0.59),差异具有统计学意义(P＜0.05);Ⅰ~Ⅱ期恶性淋巴瘤患者CD3⁺水平为(58.63±11.85),显著低于对照组的(68.96±12.63),而CD8⁺水平为(29.63±3.57),显著高于对照组的(25.67±4.53),差异具有统计学意义(P＜0.05)。结论 流式细胞仪检测外周血T细胞亚群可以作为诊断恶性淋巴瘤患者的病情、分期以及免疫状态的重要手段,具有较高的应用价值。

Clinical study of peripheral blood T cell subsets in patients with malignant lymphoma by flow cytometry

Objective The aim of this study was to investigate the effect of flow cytometry on peripheral blood T cell subsets in patients with malignant lymphoma and its relationship with clinicopathological and tumor types.Methods Ninety-eight patients with malignant lymphoma treated in our hospital from August 2014 to September 2016 were selected as the study group.Ninety-eight healthy subjects were selected as the control group.The peripheral blood T cell subsets(CD3 +,CD4 +,CD8 +,CD4 +/CD8 +)were detected in patients and healthy controls by flow cytometry.Results The levels of CD3 +and CD4 +/CD8 +in the study group were (55.63±11.25)and(1.32±0.62),respectively,which were significantly lower than those(68.96±12.63)and (1.59±0.59)of the control group(P<0.05).The levels of CD4 +and CD8 +were(33.67±8.14)and(26.02±4.67),respectively in the study group,were no difference from the control group(34.12±8.33)and(25.67±4.53)(P>0.05).The levels of CD3+and CD4 +/CD8 +in patients with Hodgkin's lymphoma were(54.63±11.36),(1.22±0.65),respectively,and(55.52±12.02),(1.34±0.71)for non-hodgkin lymphoma.They were significantly decreoseg in the control group(68.96±12.63 for CD3 +and 1.59±0.59 for CD4 +/CD8 +) (P<0.05).The level of CD4 +and CD8 +were no difference amoupst Hodgkin's lymphoma(33.78±8.23 for CD4 +and 25.74±4.88 for CD8 +),non-Hodgkin's lymphoma(25.74±4.88 for CD4 +and 33.62±8.74 for CD8 +)and control group(34.12±8.33 for CD4 +and 25.67±4.53 for CD8 +)(P>0.05).The levels of CD3 +,CD4+and CD4 +/CD8 +in patients with Ⅲ ~Ⅳ stage malignant lymphoma were(52.66±12.47), (28.25±6.32)and(1.30±0.62),respectively,which were significantly lower than those(68.96±12.63), (34.12±8.33)and(1.59±0.59)in the control group(P<0.05).The level of CD3 +in patients with phase Ⅰ-Ⅱ malignant lymphoma(58.63±11.85)was significantly lower than that in the control group(68.96±12.63)(P<0.05).The level of CD8 +in patients with phase Ⅰ-Ⅱ malignant lymphoma(29.63±3.57)was significantly higher than that in the control group(25.67±4.53)(P<0.05).Conclusion The detection of peripheral blood T cell subsets by flow cytometry can be used as an important methods to diagnose the disease,staging and immune status of patients with malignant lymphoma,which has high application value.