番茄红素对荷卵巢癌大鼠免疫功能的影响及其机制研究

目的 研究番茄红素(LP)改善荷卵巢癌大鼠免疫功能的作用并探讨其机制。方法 将卵巢癌细胞NUTU-19接种于腋窝皮下,制备荷卵巢癌大鼠模型,取60只模型大鼠随机分为模型组、顺铂组(2 mg/kg)和LP(40、20、10 mg/kg)组,每组12只;各治疗组均隔天腹腔注射给药一次,共5次。观察各组大鼠一般生存状态和肿瘤生长状况;称量肿瘤重量并计算抑瘤率,通过HE染色观察肿瘤组织形态结构变化;噻唑蓝(MTT)比色法测定脾淋巴细胞转化率;酶联免疫(ELISA)法测定血清中白细胞介素-2(IL-2)和肿瘤坏死因子-α(TNF-α)含量;流式细胞仪术(Flow cytometry)检测血中T淋巴细胞亚群(CD4⁺、CD8⁺)百分率并计算CD4⁺/CD8⁺。结果 与模型组比较,发现LP各组大鼠饮食状况好转,瘤体减小、活动度和硬度较差,肿瘤组织细胞皱缩、呈片状坏死等病变,上述改变以LP 40 mg/kg组最为显著;LP(40、20 mg/kg)组瘤重显著减轻且抑瘤率显著升高(P＜0.05,P＜0.01),脾淋巴细胞转化率显著升高(P＜0.05,P＜0.01),血清中IL-2和TNF-α含量均显著降低(P＜0.05,P＜0.01),血液中CD8⁺百分率显著降低(P＜0.05,P＜0.01),LP 40 mg/kg组CD4⁺百分率显著升高(P＜0.05),LP(40、20 mg/kg)组CD4⁺/CD8⁺显著升高(P＜0.05,P＜0.01)。结论 LP具有提高荷卵巢癌大鼠免疫功能的作用,其机制可能与LP能够有效降低炎症细胞因子(IL-2、TNF-α)含量以及提高CD4⁺/CD8⁺比值有关。

The effects and mechanism of Lycopene on immune function of ovarian cancer rats

Objective To investigate the effects of Lycopene ( LP) on immune function of ovarian cancer rats′and its mechanism .Metho ds Sixty ovarian cancer rat models made by injecting ovarian cancer cell line NUTU-19 were divided into five groups randomly:model group,Cisplatin group(2 mg/kg)and LP(40,20,10 mg/kg)group,n =12;the LP and Cisplatin were given by intraperitoneal injection ,once every two days for 5 times.The rats general states and tumor growth conditions were observed ,the tumor tissue morphological changes was observed by H&E staining;the tumor weight ,inhibition rate,spleen lymphocyte transformation rate were de -tected;and the content of IL -2 and TNF-αin serum were detected .The present of CD4 +,CD8 +in blood were detected and the ratio of CD 4 +/CD8 +was calculated .Results The food situation of the rats in LP groups was improved,tumor was reduced,activity and hardness were poorer ,necrosis presented ,tumor cell shrinkage and oth-er pathological morphological changes appeared ,especially the rats in LP 40 mg/kg group .Compared with model group,the tumor weight in LP(40,20 mg/kg)groups was decreased,the inhibition rate and the spleen lymphocyte transformation rate were increased;the contents of IL-2 and TNF-αwere decreased;the CD8+present was de-creased,while the CD4 +present in LP 40 mg/kg group was increased,the ratio of CD4 +/CD8 +was increased. All of the difference above were significant (P<0.05,P<0.01).Conclusion LP can effectively improve the immune function of ovarian cancer rat;which perhaps relates to its effects of reducing the inflammatory cytokines levels and increasing the ratio of CD 4 +/CD8 +.