OPG、RANKL在2型糖尿病伴牙周炎大鼠牙槽骨中的表达

目的:探讨骨保护素(OPG)和核因子κB受体活化因子(RANKL)在2型糖尿病伴牙周炎大鼠模型中的表达水平.方法:46只Wistar雄性大鼠,随机分为健康组10只;单纯牙周炎组12只;2型糖尿病组12只;2型糖尿病牙周炎组12只;分别建模,免疫组织化学方法检测牙槽骨OPG、RANKL蛋白表达.结果:与健康组相比,OPG在2型糖尿病组、单纯牙周炎组、2型糖尿病伴牙周炎组表达水平依次降低,RANKL的表达水平依次增强;OPG及RANKL的表达除2型糖尿病牙周炎组与单纯牙周炎组间比较无差异外,其余组间比较有统计学意义(P＜0.05).结论:炎症可能导致破骨细胞及免疫细胞中RANKL的上调和成骨细胞中OPG的下调.

OPG and RANKL expression in alveolar bone of type 2 diabetic rats with periodontitis

Objective:To investigate the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in type 2 diabetic rats with periodontitis.Methods:46 male Wistar rats were randomly divided into the healthy group(n =10),the periodontitis group(n =12),the type 2 diabetes mellitus group(n =12) and the type 2 diabetic periodontitis group(n =12).Animal models were prepared respectively.The expression of OPG and RANKL protein in alveolar bone was detected by immunohistochemistry(IHC).Results:Compared with the healthy group,the expression of OPG in the type 2 diabetes mellitus group,the periodontitis group,the type 2 diabetes mellitus with periodontitis group decreased in turn,however the expression of RANKL increased in turn.The expression of OPG and RANKL had no significant difference between periodontitis group and type 2 diabetes periodontitis group,while there was statistically significant difference among the other groups (P ＜ 0.05).Conclusion:Inflammation may lcad to upregulation of RANKL in osteoclasts and immune cells,and downregulation of OPG in osteoblasts.