调节性T细胞和Notch1信号通路在原因不明复发性自然流产中的作用

目的探讨调节性T细胞(Treg)和Notch1信号通路在原因不明复发性自然流产(URSA)中的作用。方法流式细胞仪检测URSA患者(URSA组)及正常妊娠妇女(对照组)蜕膜CD4~+CD25~+T细胞Treg表达比例,real time RT-PCR及Western blotting检测蜕膜中CD4~+T细胞中Notch1信号通路和叉头转录因子家族3(Foxp3)表达情况。结果 URSA组CD4~+CD25~+T细胞/淋巴细胞、CD4~+Foxp3~+T细胞/淋巴细胞和CD4~+Foxp3~+T细胞/CD4~+T细胞比例均低于对照组(P0.05)。URSA组CD4~+T细胞中Notch1-Ic、RBPJκ、Foxp3 m RNA及蛋白表达均低于对照组。结论 URSA患者蜕膜CD4~+T细胞中Notch1信号通路和Foxp3表达下调,CD4~+CD25~+T细胞表达比例下降,提示URSA患者Notch1信号通路和Foxp3表达下调可能阻碍CD4~+T细胞转化为CD4~+CD25~+T细胞,进而诱发免疫排斥,诱导流产。

Role of regulatory T cells and Notch1 signaling pathway in unexplained recurrent spontaneous abortion

Objective To study the role of regulatory T cells (Treg) and Notch1 signaling pathway in unexplained recurrent spontaneous abortion (URSA).Methods The proportion of CD4+CD25+T cells expression in decidua of URSA group and normal pregnancy group (control group) were analyzed by flow cytometry separately.The expression of Notchl signaling pathway and Foxp3 in decidua were detected by real time RT-PCR and Western blotting.Results In the decidua of URSA group,the proportion of CD4+CD25+T cells/lymphocyte,CD4+Foxp3+T cells/lymphocyte and CD4+Foxp3+T cells/CD4+T cells were all decreased compared with control group.The mRNA and protein of Notch 1-Ic,RBP-Jκ and Foxp3 in CD4+T cells were detected decreased significantly in URSA group than control group (P＜0.05).Conclusion In the decidua of URSA group,CD4+T cells were detected down expression of Notch1 signaling pathway and Foxp3,low proportion of CD4+CD25+T cells.Those results indicated that the reduced expression of Notch 1 signaling pathway and Foxp3 in URSA patients may block the progress of CD4+T cells transforming to CD4+CD25+T cells,then induce immunological rejection and abortion.