口腔鳞癌患者与健康人唾液差异蛋白组学的初步研究

目的:研究口腔鳞癌患者与健康人唾液蛋白差异表达情况.方法:收集口腔鳞癌患者唾液17例,健康人唾液17例,通过以双向凝胶电泳技术以及质谱鉴定和生物信息学分析,寻找在口腔鳞癌患者唾液中差异表达的蛋白质.结果:选取口腔鳞癌患者和正常人唾液中具有明显差异表达的10个蛋白质点,进行质谱鉴定和生物信息学分析,共鉴定出3种差异表达的蛋白质,其分别为S100A8、S100A8/S100A9及表皮角蛋白2(EK2)在口腔鳞癌患者唾液中均呈高表达.结论:S100A8、S100A8/S100A9及EK2可能与口腔鳞癌患者发生有关.

A primary study of the differential proteomic expression in saliva of health people and the patients with oral squamous cell carcinoma

Objective:To study the differentially expressed proteins in saliva of health people and the patients with oral squamous cell carcinoma(OSCC).Methods:Saliva of 17 cases with OSCC and paired health subjects was collected,the proteins in the saliva were examined by two-dimensional gel electrophoresis (2-DE) separation,the proteins were examined by 2-DE separation,the saliva proteome dimensional electrophoresis profiles were obtained by MALDI-TOF/MS mass spectrometric identification,the information of the differentially expressed protein in OSCC group was studied by NCBI database bioinformatics analysis.Results:10 proteins differentially expressed between the 2 groups were observed by mass spectrometry.Bioinformatics analysis showed that S100A8,S100A8/S100A9 and Epidermal cytokeratin 2(EK2) were highly expressed in the saliva of OSCC cases.Conclusion:S100A8,S100A8/S100A9 and EK2 may be related to the development of OSCC.