体外受精子代小鼠性成熟期心血管功能的评估

目的探讨体外受精(IVF)子代心血管系统的安全性。方法利用C57BL/6J小鼠建立IVF子代小鼠模型(IVF组,n=12),以C57BL/6J自然妊娠子代小鼠为对照组(n=12),观察比较小鼠性成熟期的体质量、血压,并利用彩色超声评估其心脏结构及功能。结果 12周龄IVF组子代小鼠的体质量(26.13±4.58)g]高于对照组(22.50±2.37)g],差异有统计学意义(P0.05);仔鼠的血压组间无统计学差异(P0.05)。彩色超声显示仔鼠心血管功能的主要指标(对照组,IVF组),左心室射血分数(65.41%±5.26%,65.77%±6.71%)、左室短轴缩短率(35.41%±4.05%,35.81%±4.84%)、颈动脉中内膜厚度(0.05±0.01)mm,(0.05±0.01)mm]等无统计学差异(P0.05);左房内径(2.13±0.12)mm,(2.25±0.10)mm]、左心室质量指数(8.24±0.46)g/m~2,(11.87±2.03)g/m~2]、主动脉内径(1.33±0.06)mm,(1.45±0.11)mm]3项指标IVF组显著高于对照组(P0.05)。结论 IVF子代小鼠与自然妊娠小鼠相比,部分心血管指标存在差异,提示IVF对性成熟期子代小鼠的心血管结构与功能可能存在潜在的影响,需要更多的远期随访。

Effects of in vitro fertilization on the cardiovascular function of adult offspring mice

Objective To evaluate cardiovascular health m adult mice derived from an in vitro fertilization (IVF) procedure.Methods We examined the body weight and blood pressure in adult C57BL/6J mice (12 weeks) that were conceived by IVF and natural conception (NC).Cardiovascular structure and function was evaluated by Doppler echocardiography.Results The body weight was significantly higher m IVF mice than NC mice (26.13 ± 4.58) g,(22.50 ± 2.37) g] (P＜0.05).The blood pressure was similar in the two groups.The main parameters on cardiovascular structure and function were similar between NC and IVF groups (P＞0.05),including left ventricular ejection fraction (65.41％ ± 5.26％,65.77％ ± 6.71％),left ventricular fractional shortening (35.41％ ± 4.05％,35.81％ ± 4.84％),and carotid intima-media thickness (0.05 ± 0.01) mm,(0.05 ± 0.01) mm],whereas several other parameters,such as left atrialdimension (2.13 ± 0.12)mm,(2.25 ± 0.10) mm],left ventricular mass index (8.24 ± 0.46) g/m2,(11.87 ±2.03) g/m2] and aortic dimension (1.33 ± 0.06)mm,(1.45 ± 0.11)mm],were significantly increased in IVF group compared with NC group (P＜0.05).Conclusion Mice conceived by IVF manifested a statistically significant increase in several cardiovascular structure parameters.Our findings indicate that IVF procedure may make a potential effect on cardiovascular structure and function among IVF offspring,which calls for further investigation and longer-term follow-ups.